Immunohistochemical dual staining as an adjunct in assessment of mitotic activity in melanoma

Journal of Cutaneous Pathology
Kristian IkenbergWerner Kempf

Abstract

The mitotic rate was introduced as a major prognostic criterion for the staging of thin (≤1.0 mm) melanoma by the 2009 American Joint Committee on Cancer Staging and Classification (seventh edition). The detection of a single mitotic figure changes the tumor stage in thin melanoma. We sought to address the value of a dual staining to facilitate the determination of the mitotic rate and to assign the mitotic activity to melanocytes. The mitotic rate of melanoma cells was determined by dual phosphohistone-H3 (PHH3)/Melan-A immunohistochemistry. Results were compared with PHH3 staining alone and conventional hematoxylin and eosin (H&E)-stained slides of 15 melanomas with a tumor thickness <1.0 mm. PHH3 staining clearly labeled cells in the mitotic cell cycle. The mitotic rate in the PHH3/Melan-A dual stain was equal to that derived by H&E staining. Time required for counting mitotic figures was significantly reduced. The evaluation of mitotic rate with an immunohistochemical dual stain is faster (mean 63.0%) and more reliable than evaluation by routine H&E staining alone. Dual staining immunohistochemistry may be a useful additional tool to standardize the determination of mitotic rate and may be helpful in evaluation of challengi...Continue Reading

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Citations

Nov 24, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Patricia S NielsenTorben Steiniche
Apr 6, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Michael T TetzlaffVictor G Prieto
Dec 12, 2012·Journal of Skin Cancer·Danielle R MartinezEstela E Medrano
Jan 18, 2014·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Eleonora DuregonMauro G Papotti
May 1, 2013·The American Journal of Surgical Pathology·Christopher S HaleFarbod Darvishian
Oct 6, 2012·American Journal of Surgery·Alison L BurtonGlenda G Callender
Oct 2, 2012·Dermatologic Clinics·Tammie Ferringer
Sep 23, 2014·Journal of the American Academy of Dermatology·Stevan R KnezevichJoann G Elmore
Dec 31, 2014·Archives of Pathology & Laboratory Medicine·Tammie Ferringer
Jan 21, 2017·Journal of the European Academy of Dermatology and Venereology : JEADV·C AndresN Harder
May 8, 2014·The American Journal of Dermatopathology·Ossama AbbasJag Bhawan
Jan 23, 2015·Applied Immunohistochemistry & Molecular Morphology : AIMM·Xiaoyan CuiShi Wei
Feb 14, 2017·Pathology Oncology Research : POR·Thomas M SoikeJulie Swick
Jul 3, 2013·The American Journal of Dermatopathology·Jane H ZhouVictor G Prieto

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