Immunohistochemical evaluation of H3K27 trimethylation in malignant peripheral nerve sheath tumors

Pathology, Research and Practice
Hiroshi OtsukaYoshinao Oda

Abstract

The histological definitive diagnosis of malignant peripheral nerve sheath tumor (MPNST) is quite difficult because the morphological features are not specific and no useful immunohistochemical marker has been identified. Loss-of-function mutations in EED or SUZ12, which encode the core subunit of polycomb repressive complex 2 (PRC2), were reported in MPNSTs, and the mutations were shown to cause inactivation of PRC2, leading to loss of trimethylation of histone H3 at lysine 27 (H3K27me3). Immunohistochemistry of H3K27me3 is expected to be a specific marker for MPNSTs. We evaluated immunohistochemical expression of H3K27me3 in MPNSTs with heterologous components and metachronous cases of MPNSTs. Among 145 MPNST samples, 50 (34.5%) showed complete loss of staining, and 45 (31.0%) showed partial loss of staining. Regarding the backgrounds of MPNSTs, 43 patients of neurofibromatosis type 1 (NF-1)-associated MPNST demonstrated 19 (44.2%) complete and 12 (27.9%) partial loss of H3K27me3. Among MPNSTs with heterologous component, almost all of MPNSTs with epithelioid differentiation (8/9 samples, 88.9%) retained H3K27me3, and malignant Triton tumors without epithelioid component lacked H3K27me3 at high rate (91.7%). Five of 20 metach...Continue Reading

Citations

Jun 25, 2019·Journal of Neuropathology and Experimental Neurology·Layla A AbushamatBette K Kleinschmidt-DeMasters
Jun 9, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Dylan M MarchioneJohn B Wojcik
Nov 26, 2019·Archives of Pathology & Laboratory Medicine·Cody S Carter, Rajiv M Patel
Jul 10, 2020·Neuro-oncology Advances·Anna J DareRebecca A Gladdy
Jan 18, 2020·Head and Neck Pathology·Kenichi KohashiYoshinao Oda
Nov 7, 2019·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Cody S Carter, Rajiv M Patel
Aug 17, 2020·The American Journal of Surgical Pathology·Michael E Kallen, Jason L Hornick
Sep 19, 2020·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Fei TengGuoliang Lian
May 2, 2021·Annales de pathologie·Anaïs BrunetNicolas Ortonne
Aug 28, 2021·International Journal of Molecular Sciences·Teddy MohamadJean-Philippe Brosseau

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