Immunohistochemical evaluation of stress-responsive protein sestrin2 and its correlation with p53 mutational status in eyelid sebaceous gland carcinoma

The British Journal of Ophthalmology
Perumal JayarajAnita Verma

Abstract

p53 is a stress-activated tumour suppressor gene, and its mutation has been associated with solid tumours including non-melanoma skin cancers. Sestrin2 expression is associated with DNA damage and oxidative stress and has been described as a downstream target of p53 network. However, its role in sebaceous gland carcinoma (SGC) remains unexplored. To determine the role of p53 and its downstream target gene sestrin2 expression and p53 gene mutation status in SGC. Twenty cases of eyelid SGC tumour and circulating cell-free DNA (ccfDNA) were subjected to mutational analysis of p53 gene. p53 and sesrin2 expression was evaluated by immunohistochemistry. Results were correlated with the clinicopathological features of eyelid SGC. p53 gene mutations was detected in 25% of the SGC cases. A C>T transition was identified in exon 6 in a single patient in both tumour and ccfDNA. A G>T transversion leading to amino acid change D259Y was seen in four patients. A splice site mutation affected a single case in exon 6. p53 expression was observed in 55% SGC. Loss of sestrin2 in 55% SGC cases correlated with poor tumour differentiation (P=0.0001), upper eyelid involvement (P=0.004), p53 mutation (P=0.039) and with mutant p53 expression (P=0.0001)...Continue Reading

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Citations

Aug 17, 2019·Current Molecular Medicine·Sacha Nahon-EstevePaul Hofman
Dec 24, 2019·Frontiers in Immunology·Li-Xue WangYong-Ming Yao
Dec 8, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jie Wang BJunlian Gu

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