PMID: 9163873May 1, 1997Paper

Immunohistochemical identification of caprine arthritis-encephalitis virus in paraffin-embedded specimens from naturally infected goats

Veterinary Pathology
Anne K StorsetEspen Rimstad

Abstract

The expression of caprine arthritis-encephalitis virus capsid protein was studied in seropositive naturally infected asymptomatic goals (10< seropositive naturally infected encephalitic kids (12) and goats (4), and noninfected control goats (3). Rabbit antiserum to recombinant viral capsid and matrix proteins were used in a biotin-streptavidin-alkaline phosphatase complex immunohistochemical method on sections of formalin- and ethanol-fixed tissue specimens. Macrophages in inflamed areas of the lung (8/12), in the brain (5/16), and in the spinal cord (4/16) from encephalitic animals harbored viral antigens, as revealed by immunohistochemistry and use of a capsid protein-specific antiserum. Altogether 12/16 encephalitic animals tested positive for viral antigen. Viral antigens were found in 5/10 seropositive asymptomatic goals in macrophages located in the lung (3), the udder (1), and the medulla of lymph nodes (4). None of the control animals tested positive for viral antigen. Ethanol fixation showed highest sensitivity, and the lowest antigen concentration that revealed a positive signal discernible from background was twofold higher in ethanol-fixed specimens than in formalin-fixed specimens. The evaluation was performed on a...Continue Reading

References

Sep 1, 1992·The Journal of Infectious Diseases·S J BrodieJ C DeMartini
Nov 1, 1992·Veterinary Microbiology·R G ZanoniE Peterhans
Oct 1, 1985·Journal of Comparative Pathology·S Kennedy-StoskopfJ D Strandberg
Feb 1, 1974·The Journal of Infectious Diseases·L C CorkR C Piper
Feb 29, 1980·Science·T B CrawfordL C Cork
Jan 1, 1984·Journal of Comparative Pathology·W F Robinson, T M Ellis
May 1, 1980·The Histochemical Journal·P PetruszJ C Finley
Jan 1, 1994·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·O EvensenA Nilsen
Mar 1, 1994·Virus Genes·N Carey, R G Dalziel
Jan 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Peter Petrusz

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Citations

Oct 17, 2008·Journal of Comparative Pathology·J BenavidesV Pérez
Nov 7, 2008·Veterinary Microbiology·Britt GjersetChristine Monceyron Jonassen
Mar 2, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kristin M PattonBeth A Valentine
Nov 23, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Lynn M Herrmann-Hoesing
Sep 5, 2020·Animals : an Open Access Journal From MDPI·Izabella DolkaJarosław Kaba

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