Mar 13, 2008

Immunohistochemical identification of MMP-2 and MMP-9 in human dentin: correlative FEI-SEM/TEM analysis

Journal of Biomedical Materials Research. Part a
Annalisa MazzoniLorenzo Breschi

Abstract

Matrix metalloproteinases (MMPs) are a family of peptidases trapped within mineralized dentin matrix and involved with degradation of the extracellular matrix components in hybrid layers and caries. Despite their identification through indirect evidences and biochemical assays, MMP-2 and -9 have not been localized within the human dentin extracellular organic matrix. Thus, this study aimed to assess the localization and distribution of MMP-2 and -9 in human dentin organic matrix by employing a correlative field emission in-lens-scanning electron microscopy (FEI-SEM) and transmission electron microscopy (TEM) immunohistochemical approach. Dentin specimens were submitted either to a preembedding or to a postembedding immunolabeling technique using primary monoclonal antibodies anti-MMP-2 and anti-MMP-9 and exposed to a secondary antibody conjugated with gold nanoparticles. MMP-2 and -9 labelings were identified in the demineralized dentin matrix as highly electron-dense gold particles dispersed on the collagen fibrils. Correlative FEI-SEM/TEM observations confirmed that MMP-2 and MMP-9 are endogenous components of the human dentin organic matrix and revealed the three-dimensional relationship between these proteinases and the col...Continue Reading

  • References30
  • Citations47

References

  • References30
  • Citations47

Citations

Mentioned in this Paper

Establishment and Maintenance of Localization
Monoclonal Antibodies
Monoclonal antibodies, antineoplastic
Extracellular
Enzymes, antithrombotic
Peptide Hydrolases
Extracellular Matrix
Matrix Metalloproteinase 2
Immunocytochemistry
Endopeptidases

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