Immunohistochemical identification of neurons in ganglia of the guinea pig sphincter of Oddi

The Journal of Comparative Neurology
D G WellsGary M Mawe

Abstract

The sphincter of Oddi is a smooth muscle sphincter that regulates the flow of bile into the duodenum. To identify potential chemical coding in sphincter of Oddi neurons, immunohistochemistry and histochemistry were employed to assay for putative neurotransmitters and related synthetic enzymes in wholemount preparations, with and without colchicine treatment. Immunoreactivities for enkephalin-endorphin (ENK-END), substance P (SP), nitric oxide synthase, vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), and calcitonin gene-related peptide (CGRP) were demonstrated within the ganglionated plexus. Roughly half of the neurons in the sphincter of Oddi expressed immunoreactivity for both SP and ENK-END, but not for nitric oxide synthase. About 25% of the neurons expressed nitric oxide synthase immunoreactivity as well as NADPH-diaphorase activity. This contingent of neurons was made up of two subgroups: one that expressed immunoreactivity for VIP, the other for NPY. Neurons that expressed CGRP immunoreactivity were sparse in sphincter of Oddi ganglia; however, many axons immunoreactive for both CGRP and SP were present in the ganglionated plexus. The CGRP/SP fibers are probably visceral afferents that may influence ganglionic ...Continue Reading

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Citations

Mar 16, 2007·Digestive Diseases and Sciences·Antonio Bosch, Luis R Peña
Sep 12, 2000·Current Gastroenterology Reports·A L KennedyG M Mawe
May 12, 1997·Journal of the Autonomic Nervous System·E K TalmageG M Mawe
Feb 24, 2001·Journal of Autonomic Pharmacology·F Vogalis, T K Smith
Jun 6, 1998·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M R CoxG T Saccone
Jul 11, 1998·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·K Hillsley, G M Mawe
Jan 1, 2013·Polish Journal of Veterinary Sciences·O Tomaszewska, J Kaleczyc
Sep 24, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Onesmo B BalembaGary M Mawe
Sep 11, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J D Wood, J J Galligan
Aug 20, 2005·Pharmacological Reviews·Noboru Toda, Arnold G Herman
Jul 17, 1999·The American Journal of Physiology·G M Mawe, A L Kennedy
Jul 12, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·B P Manning, G M Mawe
Nov 14, 1998·The American Journal of Physiology·K Hillsley, G M Mawe

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