Immunohistochemical identification of the cytoskeletal elements in the notochord cells of bony fishes

Journal of Morphology
R J Schmitz

Abstract

The medulla of the unconstricted notochords of the shortnose sturgeon, Acipenser brevirostratus, and African lungfish, Protopterus annectens, and the cellular component of the intervertebral joint tissue of the teleost fish, Perca flavescens, are comprised of cells with a large central vacuole. Previous studies on the fine structure of this tissue revealed that the cytoplasm surrounding these vacuoles consists of 10-nm-diameter intermediate filaments. Since in mammals there are a large number of tissue-specific types of intermediate filaments, this study uses antibodies to mammalian intermediate filaments to determine the type of filaments present in the notochord cells of bony fishes. Positive labeling using a polyclonal antibody to human skin keratins is observed in the cytoplasm of the notochord cells in the intervertebral tissues of Perca. These tissues are also probed with the AE series antibodies that label keratins found in mammalian epithelial cells. In both Protopterus and Acipenser the peripheral cytoplasm of the notochord cells is labeled with all three AE antibodies. In Perca only the AE3 antibody probe produces positive staining. These staining patterns are consistent with previous studies on the localization of cy...Continue Reading

References

Jan 1, 1991·Acta histochemica·U WelschI C Potter
Jan 1, 1991·Cell Motility and the Cytoskeleton·O Skalli, R D Goldman
Feb 1, 1990·Current Opinion in Cell Biology·M Stewart
Jan 1, 1985·Annals of the New York Academy of Sciences·T T SunR Eichner
Jan 1, 1985·Annual Review of Cell Biology·P M Steinert, D A Parry
Jul 1, 1988·Genes & Development·S E LaFlammeI B Dawid
Feb 1, 1989·Current Opinion in Cell Biology·R M Robson
Jan 1, 1988·Annual Review of Biochemistry·P M Steinert, D R Roop
Jan 1, 1985·Annals of the New York Academy of Sciences·R J LasekL Autilio-Gambetti
Nov 1, 1982·The Journal of Cell Biology·J Woodcock-MitchellT T Sun
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E Fuchs, D Marchuk
Sep 1, 1993·Veterinary Pathology·T E Bunton
Feb 7, 1997·Cell·S J MorrisonD J Anderson

❮ Previous
Next ❯

Citations

Feb 24, 2004·Journal of Hematotherapy & Stem Cell Research·María del Carmen Cárdenas-AguayoLuis Covarrubias
Jul 11, 2006·Micron : the International Research and Review Journal for Microscopy·C Di PrimioM Mazzini
May 15, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Di Jiang, William C Smith
Jun 7, 2007·Journal of Cell Science·Susan J NixonRobert G Parton
Jun 17, 2021·PLoS Computational Biology·Misaki SakashitaShigeru Kondo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.