Immunohistochemical investigation of dopaminergic terminal markers and caspase-3 activation in the striatum of human methamphetamine users.

International Journal of Legal Medicine
Osamu KitamuraShin-ichi Kubo

Abstract

Methamphetamine (METH) has been shown to induce neurotoxicity. In a previous human study using quantitative Western blotting and radioligand binding assay, dopaminergic terminal marker deficits were induced in chronic METH users. In this study, we examined the suitability of the immunohistochemical detection of tyrosine hydroxylase (TH), dopamine transporter (DAT), and vesicular monoamine transporter-2 (VMAT2) levels, and caspase-3 activation in the striatum to diagnose METH abuse. Decreases in TH immunoreactivity in the nucleus accumbens and DAT in the nucleus accumbens and putamen were induced in METH users, whereas a significant difference of VMAT2 was not evident between METH and control groups. However, in the nucleus accumbens of two METH users, levels of VMAT2, a stable marker of striatal dopaminergic terminal integrity, were reduced remarkably. These findings might indicate that dopaminergic terminal degeneration is induced in the striatum of some METH abusers. On the other hand, we observed little caspase-3 activation, indicative of apoptosis, in the striatal neurons of chronic METH users. Overall, the findings of dopaminergic terminal markers were similar to those in the previous human study. Therefore, it is suggeste...Continue Reading

References

Oct 1, 1989·Neuropharmacology·M J De Vito, G C Wagner
Dec 29, 1995·European Journal of Pharmacology·T Vander BorghtK Frey
May 23, 1998·Neurochemistry International·J L Cadet, C Brannock
Jun 11, 1999·The Journal of Comparative Neurology·B J CiliaxA I Levey
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Allen M Gown, Mark C Willingham
Jul 17, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·David S SegalArthur K Cho
Oct 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jean Lud CadetXiaolin Deng
Nov 20, 2003·International Journal of Legal Medicine·R HausmannP Betz
Dec 9, 2003·European Journal of Pharmacology·Kamisha L Johnson-DavisDiana G Wilkins
Jan 7, 2004·Biological Psychiatry·Hideo MatsuzakiKohji Sato
Feb 11, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Subramaniam JayanthiJean Lud Cadet
Jun 11, 2004·Journal of Neurochemistry·Anna MireckiStephen J Kish
Apr 28, 2005·International Journal of Legal Medicine·J DresslerA Busuttil
Aug 2, 2005·International Journal of Legal Medicine·R HausmannP Betz

❮ Previous
Next ❯

Citations

Oct 29, 2011·The Journal of Pharmacology and Experimental Therapeutics·Lisa M McFaddenAnnette E Fleckenstein
Feb 1, 2014·Legal Medicine·Hitoshi MaedaTomomi Michiue
Sep 28, 2013·Neurotoxicity Research·Carlos D SilvaFrederico C Pereira
Sep 1, 2015·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Isabelle BoileauStephen J Kish
Aug 20, 2010·Forensic Science International : Synergy·Hitoshi MaedaTomomi Michiue
Jan 30, 2010·Legal Medicine·Osamu KitamuraShin-ichi Kubo
Mar 31, 2009·Brain Research Reviews·Irina N Krasnova, Jean Lud Cadet
Mar 6, 2016·The International Journal of Neuropsychopharmacology·Liliana MendietaRosario Moratalla
Dec 21, 2012·Neuroscience and Biobehavioral Reviews·Scott Mackey, Martin Paulus
Apr 8, 2014·Neurobiology of Disease·Junchao TongStephen J Kish
Oct 9, 2008·Psychopharmacology·Stephen J KishJunchao Tong
Jan 11, 2017·The Journal of Toxicological Sciences·Wanida TungkumJiraporn Tocharus
Jun 21, 2017·The Journal of Pharmacology and Experimental Therapeutics·Anna Moszczynska, Sean Patrick Callan
Dec 22, 2017·CNS Neuroscience & Therapeutics·António Pedro Delgado MoraisFrederico Costa Pereira
Oct 7, 2021·Applied Biochemistry and Biotechnology·Sara NikeafsharReza Tahvilian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis