Immunohistochemical localization of aquaporin 10 in the apical membranes of the human ileum: a potential pathway for luminal water and small solute absorption

Histochemistry and Cell Biology
A MobasheriD Marples

Abstract

A new member of the aquaporin family (AQP10) has recently been identified in the human small intestine by molecular cloning and in situ hybridization. Ribonuclease protection assay and northern blotting have demonstrated that AQP10 is expressed in the human duodenum and jejunum. However, the subcellular distribution of the AQP10 protein and its plasma membrane polarization have not yet been established. The objective of this study was to determine the distribution of the AQP10 protein in the human ileum by immunohistochemistry and western blotting using a polyclonal antibody raised against a unique 17-amino acid peptide derived from the human AQP10 sequence. The distribution of the AQP1 and AQP3 proteins was also studied by immunohistochemical staining using affinity-purified polyclonal antibodies. Results revealed that the AQP10 protein is preferentially targeted to the apical membrane domain of absorptive intestinal epithelial cells, whereas AQP3 is located in the basolateral membrane of the cells and AQP1 expression is restricted to the mucosal microvascular endothelia. The presence of AQP10 in the apical membrane of intestinal villi suggests that this protein may represent an entry pathway for water and small solutes from t...Continue Reading

Citations

Aug 26, 2009·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Miwa Suzuki
Jul 8, 2011·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Jonathan M Whittamore
Dec 23, 2004·Histochemistry and Cell Biology·Esther Asan, Detlev Drenckhahn
Jul 8, 2011·The Journal of Biological Chemistry·Fredrik ÖbergKristina Hedfalk
Dec 7, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Simona TrittoMarina Zelenina
Oct 27, 2007·Annual Review of Physiology·Aleksandra RojekRobert A Fenton
Aug 6, 2008·International Journal of Environmental Research and Public Health·Hari H P CohlyRajendram V Rajnarayanan
Dec 18, 2013·Journal of Mammary Gland Biology and Neoplasia·Ali Mobasheri, Richard Barrett-Jolley
Jul 11, 2014·Molecular and Cellular Endocrinology·Leire Méndez-GiménezGema Frühbeck
Dec 17, 2008·Clinical and Experimental Nephrology·Kenichi IshibashiShintaro Kondo
Apr 3, 2012·Molecular Aspects of Medicine·Umberto Laforenza
Jun 10, 2005·Biology of the Cell·Umberto LaforenzaUlderico Ventura
Nov 5, 2005·Alimentary Pharmacology & Therapeutics·C SchillerW Weitschies
Sep 19, 2006·British Journal of Pharmacology·C L Hirota, D M McKay
Apr 27, 2010·Biology of the Cell·Umberto LaforenzaGino R Corazza
Aug 23, 2012·Molecular Membrane Biology·Fredrik Oberg, Kristina Hedfalk
Aug 28, 2015·PloS One·Alexandre P GarneauPaul Isenring
Jul 30, 2016·International Journal of Molecular Sciences·Alessandra PelagalliRosaria Meli
Jul 28, 2016·International Journal of Molecular Sciences·Mariusz T SkowronskiSøren Nielsen
Sep 3, 2016·International Journal of Molecular Sciences·Cui ZhuZongyong Jiang
Sep 28, 2016·Molecular Nutrition & Food Research·Thérèse SergentYves-Jacques Schneider
Feb 26, 2010·Rheumatology·Masashizu NagaharaTakanobu Otsuka
Jan 13, 2010·Reproductive Sciences·Rachel V FloydAli Mobasheri
Mar 10, 2018·The Journal of Pathology·Ravi Kiran DeeviFrederick Charles Campbell
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Megumi IshiiHiroaki Yuasa
Jul 26, 2015·Applied and Environmental Microbiology·Xiao-Dan LiuShi-Hong Zhang
Sep 26, 2007·Journal of Neuroscience Research·Mia M ThiMenachem Hanani
Aug 4, 2019·Pharmaceuticals·Maryam NakhjavaniJennifer E Hardingham
Sep 26, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Zhiyuan MaTaolang Li
Aug 14, 2018·Physiological Reports·Caragh WalpoleGavin Stewart
Mar 15, 2006·World Journal of Gastroenterology : WJG·Shun-Ying JinTong-Hui Ma
Sep 12, 2007·Journal of Proteome Research·Mark DonowitzXuhang Li
Jan 5, 2012·Chemical Research in Toxicology·Marta CalatayudVicenta Devesa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.