Immunohistochemical localization of brain-derived neurotrophic factor in adult rat brain

Neuroscience
Y KawamotoJ Kimura

Abstract

To investigate the role of brain-derived neurotrophic factor in the central nervous system, we produced an anti-peptide antibody that specifically recognized brain-derived neurotrophic factor and performed immunohistochemistry for brain-derived neurotrophic factor-like immunoreactivity in normal adult rat brain. A synthetic peptide (EKVPVSKGQL), derived from mature brain-derived neurotrophic factor, was conjugated to bovine thyroglobulin at a ratio of 1:3 and used as an immunogen to produce a high-titre anti-brain-derived neurotrophic factor polyclonal antibody in Japanese white rabbits. Dot blotting demonstrated that the antiserum could detect 3.91 pmol of synthetic peptide, and Western blotting showed that the antiserum recognized one band with a molecular weight consistent with that of brain-derived neurotrophic factor. In immunohistochemistry, brain-derived neurotrophic factor-like immunoreactivity was widespread in adult rat brain, including cerebral cortex, hippocampus, basal forebrain, striatum, hypothalamus, brainstem and cerebellum. Not only neuronal somata but also nerve fibres showed positive staining. Our data suggest that brain-derived neurotrophic factor is transported through axons in a subpopulation of neurons i...Continue Reading

References

May 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kazuharu FurutaniMasamitsu Iino
Sep 26, 2006·The Cerebellum·Rosemarie Drake-Baumann
May 23, 2008·Chemical Senses·Amy C ClevengerDiego Restrepo
Apr 16, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kirk I EricksonArthur F Kramer
Sep 9, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A Hafidi
Aug 1, 2015·Nutritional Neuroscience·Yong Jun JinMei Zi Yang
Oct 13, 2005·Journal of Chemical Neuroanatomy·Ken TakumiMotoharu Hayashi
Dec 6, 2016·International Journal of Molecular Sciences·Beatriz Benítez-TemiñoAngel M Pastor
Aug 23, 2005·Brain Research. Brain Research Reviews·Beatriz Benítez-TemiñoAngel M Pastor
Aug 7, 2001·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·H B TreloarC A Greer
Mar 19, 2014·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Tin-Yun HoChing-Liang Hsieh
Aug 11, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hong-Tian ZhangTim Ting-Hua Wang
Mar 14, 2002·The Journal of Comparative Neurology·Beatriz Benítez-TemiñoAngel M Pastor
Apr 30, 2004·The Journal of Comparative Neurology·Beatriz Benítez-TemiñoAngel M Pastor
Dec 7, 2006·Journal of Neuroscience Research·Tamara AidTõnis Timmusk
Jul 22, 1998·Nature·L M Machesky, M Way
Jul 1, 1997·Reviews in the Neurosciences·M DragunowR L Faull
Aug 28, 2020·Cell and Tissue Research·S MeisVolkmar Lessmann
Dec 14, 2018·Experimental and Therapeutic Medicine·Zhenbo LiuLiguo Qi
Jul 29, 2017·Expert Review of Ophthalmology·B A MysonaK E Bollinger
Mar 8, 2012·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Makoto TakahashiHidehiro Mizusawa
Nov 2, 2014·Biological Research for Nursing·Elizabeth D E PapathanassoglouMaria N Karanikola
Jan 27, 2015·Addiction Biology·Dolores Vazquez-SanromanMarta Miquel

Citations

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·U PirvolaM Saarma
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C A AltarC Hyman
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C J WetmoreL Olson
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S CeccatelliT Hökfelt
Jan 15, 1991·Biochemical and Biophysical Research Communications·Y KaishoK Igarashi
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B KnüselF Hefti
Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Aug 1, 1994·The European Journal of Neuroscience·S D CrollH Nawa
Feb 1, 1994·Hearing Research·L C Schecterson, M Bothwell
Jul 30, 1993·Biochemical and Biophysical Research Communications·H OkazawaI Kanazawa
Apr 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C J WetmoreL Olson
Sep 1, 1995·The European Journal of Neuroscience·M M Dugich-DjordjevicF Hefti
Jul 1, 1991·The European Journal of Neuroscience·Wilma J. FriedmanHåkan Persson

Related Concepts

Establishment and Maintenance of Localization
Recombinant Thyroglobulin
Immunohistochemistry
Entire Brainstem
Immunoreactivity
Neostriatum
Western Blotting
Neurons
Brain
Recombinant Brain-Derived Growth Factors

Related Feeds

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.

Basal Forebrain & Food Avoidance

Neurons in the basal forebrain play specific roles in regulating feeding. Here are the latest discoveries pertaining to the basal forebrain and food avoidance.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.