PMID: 6408932Jul 1, 1983Paper

Immunohistochemical localization of carbonic anhydrase in postnatal and adult rat kidney

The American Journal of Physiology
D BrownL Orci

Abstract

With use of specific antibodies against human and rat erythrocyte carbonic anhydrase C and human carbonic anhydrase B, only the isozyme C could be detected by immunofluorescence in rat kidney epithelial cells. In the postnatal kidney a few cells were positive after 2 days, but the number of fluorescent cells increased during the first few weeks of life to reach the final adult levels after 3 wk in the cortex and 5 wk in the medulla. In the postnatal and adult kidney a characteristic mosaic pattern of fluorescence was seen in the late distal tubule, the connecting segment, and the collecting tubule, where the mitochondria-rich dark cells were brightly fluorescent. In addition, in later postnatal stages and in the adult, the entire epithelium of the initial portion of descending thin limbs of Henle (long loops) was labeled. Some kidney regions that had previously been shown to contain carbonic anhydrase activity by biochemical and histochemical techniques stained only weakly with the immunocytochemical method. This suggests either that the enzyme in these regions does not cross-react strongly with our antibodies or that these regions contain only low amounts of carbonic anhydrase, at the limit of the detection threshold of our te...Continue Reading

Citations

Apr 9, 2001·American Journal of Physiology. Renal Physiology·C A WinklerG J Schwartz
Mar 12, 2004·American Journal of Physiology. Cell Physiology·Teodor G PaunescuDennis Brown
Aug 22, 2014·American Journal of Physiology. Renal Physiology·David S HainsAndrew L Schwaderer
Feb 22, 2001·American Journal of Physiology. Renal Physiology·C BagnisD Brown
May 12, 2020·American Journal of Physiology. Renal Physiology·John KetzAndrew L Schwaderer
Aug 11, 2017·American Journal of Physiology. Renal Physiology·Chao GaoWenzheng Zhang
Apr 5, 2020·Biomolecules·Jacob AndringRobert McKenna
Jun 1, 2006·American Journal of Physiology. Renal Physiology·Wan-Young KimJin Kim

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