PMID: 7540756Mar 1, 1995Paper

Immunohistochemical localization of endothelial nitric oxide synthase in human villous and extravillous trophoblast populations and expression during syncytiotrophoblast formation in vitro

Placenta
A L EisL Myatt

Abstract

We have examined the distribution of the endothelial isoform of nitric oxide synthase (eNOS) in villous and extravillous trophoblast populations by immunohistochemistry and have further studied expression of eNOS during differentiation of cytotrophoblast into syncytiotrophoblast in culture. In first trimester villous tissue, NADPH diaphorase activity and eNOS immunostaining were present in syncytiotrophoblast but not the progenitor cytotrophoblast layer. Extravillous trophoblast in the basal plate of the placenta was identified by anticytokeratin immunostaining and displayed NADPH diaphorase activity, but not eNOS immunostaining. Both amnion epithelial cells and chorion cytotrophoblast had NADPH diaphorase activity but no eNOS immunostaining, whereas eNOS immunostaining was seen in the fibroblast layer of amnion. Purified villous cytotrophoblast cells from term placentae aggregated and fused to form a syncytium with increasing time in culture as assessed by antidesmosomal protein and antinuclear antibody immunostaining. Following 24 h in culture, the majority of cells were still mononucleate cytotrophoblast which did not display eNOS immunostaining, whereas a few syncytial aggregates had formed which were both eNOS positive and...Continue Reading

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Citations

Jul 13, 2004·Histochemistry and Cell Biology·Leslie Myatt, Xiaolan Cui
Jan 1, 1996·Pharmacology & Therapeutics·J Norman
May 11, 1999·The American Journal of Pathology·F LyallS C Robson
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Feb 13, 2001·Placenta·J F DyeJ A Firth
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Apr 4, 2017·The Journal of Clinical Endocrinology and Metabolism·Jie XuYuping Wang
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Jul 31, 2013·Journal of Perinatal Medicine·Piya ChaemsaithongTinnakorn Chaiworapongsa
Apr 17, 2003·The Journal of Obstetrics and Gynaecology Research·Kazuo AsagiriTakafumi Kudo

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