Immunohistochemical localization of ghrelin in rodent kidneys

Histochemistry and Cell Biology
Akira YabukiShusaku Suzuki

Abstract

Ghrelin is a novel peptide hormone, originally identified in the rat and human stomach that plays various important roles. In the present study, we report the intra-renal localization of ghrelin in laboratory rodents. Kidneys from 3 month-old mice, rats and hamsters of both sexes were analyzed by immunohistochemistry. Positive signals were clearly observed in the epithelium of the distal tubules, whereas other segments of the nephron or interstitial cells, including juxtaglomerular cells, showed negative reactions. Pre-embedding immunoelectron microscopy revealed positive signals exclusively on the basolateral membrane in the distal tubular cells and in the collecting ducts. In addition, prepro-ghrelin gene expression was assessed by RT-PCR, and the expected 329-bp prepro-ghrelin mRNA was clearly detected in the kidney. On Western blot analysis, although a specific band for ghrelin (3 kDa) was not detected in the kidney, the expected band for prepro-ghrelin (13 kDa) was clearly detected in both the stomach and the kidney. This paper clarified the intra-renal localization of ghrelin.

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Citations

Dec 7, 2006·Histochemistry and Cell Biology·Douglas J TaatjesJürgen Roth
Nov 1, 2007·Histochemistry and Cell Biology·Christian ZuberJürgen Roth
Mar 8, 2008·Endocrine Journal·Simoni A KatergariNikos K Nikolettos
Dec 23, 2006·Biochemical and Biophysical Research Communications·Aihua LiAndrzej S Tarnawski
Mar 27, 2009·Microscopy Research and Technique·Nadia ArcamoneFinizia Russo
Mar 19, 2015·Peptides·Bilge AygenIbrahim Hanifi Ozercan
May 5, 2017·International Journal of Molecular Sciences·Joanna BoniorJolanta Jaworek
Jul 11, 2009·The Journal of International Medical Research·B AygenH Celiker
Oct 21, 2009·Renal Failure·Tuncay Kuloglu, Durrin Ozlem Dabak
Aug 11, 2015·The Journal of Veterinary Medical Science·Akira YabukiOsamu Yamato
Mar 15, 2018·Acta histochemica·Ahmed E NoreldinYoshinao Z Hosaka

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