PMID: 9429077Jan 16, 1998Paper

Immunohistochemical localization of heparin-binding epidermal growth factor-like growth factor in normal skin and skin cancers

The Histochemical Journal
M T DowningG E Besner

Abstract

Heparin-binding epidermal growth factor (EGF)-like growth factor is a 22-kDa glycoprotein that was originally identified as a secreted product of cultured human macrophages. Although the growth factor mRNA has been identified in various cells and tissues, the tissue distribution of the protein itself has rarely been demonstrated. In this study, the EGF-like growth factor was detected immunohistochemically in a variety of human skin samples by indirect immunofluorescence using a polyclonal rabbit antiserum raised against residues 26-41 of mature heparin-binding EGF. The keratinocytes of a variety of epithelium-derived structures demonstrated reproducible, specific staining for the EGF. In normal tissues, this staining was prominent in the basal cells of the epidermis and in the epithelial cells lining epidermal appendages such as hair follicles, sebaceous sweat glands and eccrine sweat glands. In addition, specific staining was detected in skin cancers derived from the basal epithelial cell layer, including basal and squamous cell carcinomas of the skin, with no staining detected in melanoma specimens. Immunoreactive heparin-binding EGF was characteristically associated with the surface of cells. With minor exceptions, the immun...Continue Reading

Citations

Dec 19, 1998·Microscopy Research and Technique·L G Hudson, L J McCawley
Mar 30, 2001·The Journal of Biological Chemistry·L StrachanK D Kumble
Jun 22, 2012·Medical Molecular Morphology·Suguru DateokaKenji Kakudo
Mar 8, 2002·The Journal of Burn Care & Rehabilitation·Randolph K CribbsGail E Besner
Apr 26, 2003·The Journal of Biological Chemistry·Kingston K L Mak, Siu Yuen Chan
Oct 26, 2016·Annals of Plastic Surgery·Hilmi ŞenUğur Koçer
Jun 13, 2017·Annals of Plastic Surgery·Kadri Ozer, Özlem Çolak
Sep 13, 2007·Annual Review of Pharmacology and Toxicology·Claude BackendorfMathieu Noteborn
Aug 4, 2004·Cancer Research·Pat P OngusahaSam W Lee
May 26, 2007·The American Journal of Pathology·Laure RittiéJames T Elder
Nov 25, 2006·The Journal of Investigative Dermatology·Martina I OkwuezeLillian B Nanney

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