Immunohistochemical localization of inducible and endothelial nitric oxide synthase in porcine ovaries and effects of NO on antrum formation and oocyte meiotic maturation

Molecular and Cellular Endocrinology
Yong TaoHuirong Xie

Abstract

The present study is to investigate the immunolocalization of endothelial and inducible nitric oxide synthase (eNOS, iNOS) in porcine ovary and the effect of nitric oxide (NO) on antrum formation and oocyte meiotic resumption. In Experiment 1, preantral follicles (250-300 microm in diameter) were cultured in 0 (Control), 0.1, 0.3, 0.5 or 1 mM sodium nitroprusside (SNP), a NO donor. In Experiment 2, the cumulus-oocyte complexes (COCs) aspirated from medium follicles (3-6 mm in diameter) were incubated in 0.1mM SNP or two inhibitors for NOS, 10 mM aminoguanidine bicarbonate salt (AG) or 1 mM Nomega-nitro-l-arginine methyl ester (L-NAME), alone or concomitantly. In Experiment 3, ovarian tissues, corpus luteum (CL), corpus albican (CA) and COCs from small (1-2 mm in diameter), medium (3-6 mm) and large follicles (7-10 mm) were isolated, rinsed, fixed, paraffin embedded and stained by the conventional avidin-biotin complex method for the detection of eNOS and iNOS production. The results showed that 0.1mM SNP had no effect on antrum formation (P > 0.05) while 0.3, 0.5 or 1 mM significantly inhibited the antrum formation (P < 0.05). AG markedly inhibited porcine oocyte meiotic resumption (P < 0.05) while L-NAME inhibited first polar ...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S LamasT Michel
Sep 1, 1992·Biology of Reproduction·D E MorbeckJ H Britt
Nov 1, 1984·Journal of Reproduction and Fertility·J MotlikJ Fulka
Sep 23, 1994·Cell·C Nathan, Q W Xie
May 1, 1994·Journal of Reproduction and Fertility·H FunahashiB N Day
Aug 1, 1997·Nitric Oxide : Biology and Chemistry·S Dimmeler, A M Zeiher
Jun 11, 1998·Human Reproduction Update·M RosselliR K Dubey
Apr 17, 1999·Journal of the Society for Gynecologic Investigation·A Jablonka-ShariffL M Olson
Feb 5, 2000·Journal of Molecular Endocrinology·M A HattoriN Fujihara
Mar 1, 2000·Molecular Reproduction and Development·A Jablonka-Shariff, L M Olson
Oct 12, 2000·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·N NishidaN Fujihara
Feb 22, 2001·Animal Reproduction Science·V D Dixit, N Parvizi
Aug 24, 2001·Domestic Animal Endocrinology·F GrasselliC Tamanini
Dec 6, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·A NishikimiM Yamada
Jan 10, 2002·Fertility and Sterility·Lukas A Hefler, Anthony R Gregg
Jun 8, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·G ShuttleworthM G Hunter
Oct 23, 2002·Biology of Reproduction·Yasuhiko NakamuraHiroshi Kato
Feb 7, 2003·Current Pharmaceutical Design·Catherine D Thaler, David Epel
Sep 16, 2003·Molecular and Cellular Endocrinology·Shumin BuBo Zhou
Feb 18, 2004·The Journal of Reproduction and Development·Katsuhisa TakesueMasa-aki Hattori

❮ Previous
Next ❯

Citations

Jul 3, 2013·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Kerong ShiNing Li
Jan 30, 2010·Zygote : the Biology of Gametes and Early Embryos·Eva ChmelíkováFrantisek Jílek
Mar 24, 2012·Animal : an International Journal of Animal Bioscience·H TichovskáR Rajmon
Jul 2, 2008·Molecular Human Reproduction·Jonathan Van BlerkomVerena Thalhammer
Mar 31, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Li-Jun HuoQing-Yuan Sun
Jan 2, 2009·The European Journal of Contraception & Reproductive Health Care : the Official Journal of the European Society of Contraception·Sevtap KilicSertac Batioglu
Jun 22, 2005·Molecular and Cellular Endocrinology·Jaroslav PetrFrantisek Jílek
Jan 25, 2011·Molecular and Cellular Endocrinology·Gustavo ZamberlamChristopher A Price
Aug 23, 2012·PloS One·Michal JesetaJean-François Bodart
Oct 17, 2016·Theriogenology·D F DubeibeC S Paes de Carvalho
Nov 7, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Vinay Kumar Singh, Bechan Lal
Jun 28, 2017·Zygote : the Biology of Gametes and Early Embryos·Ramon Cesar BotigelliClaudia Lima Verde Leal
Jul 22, 2019·Journal of Assisted Reproduction and Genetics·Florentin-Daniel StaicuCarmen Matás Parra
Aug 26, 2009·Communicative & Integrative Biology·Linda LefièvreSteve Publicover
Jan 28, 2021·International Journal of Environmental Research and Public Health·Maria Cristina Budani, Gian Mario Tiboni
Jan 3, 2021·Research in Veterinary Science·Jon Romero-AguirregomezcortaCarmen Matás

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.