Immunohistochemical localization of insulin-degrading enzyme along the rat intestine, in the human colon adenocarcinoma cell line (Caco-2), and in human ileum

Journal of Pharmaceutical Sciences
L L ChangJ P Bai

Abstract

Insulin-degrading enzyme (IDE) has been implicated in the intracellular degradation of insulin in insulin target cells. Knowledge of the existence of this enzyme in the intestine will be beneficial to the achievement of clinical oral efficacy of insulin. A comparative study was conducted with rat intestine, human colon adenocarcinoma (Caco-2) cells, and human ileum. Confocal microscopy analysis using the anti-IDE antibody showed that IDE was localized in the mucosal cells of rat and human intestines, as well as in Caco-2 cells. Immunostaining of this enzyme was homogeneous throughout the cell excluding nucleus, indicating a typical cytosolic distribution in rat and human enterocytes and in Caco-2 cells.

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Citations

Jul 6, 2000·International Journal of Pharmaceutics·M MorishitaT Nagai
Nov 1, 1996·The Journal of Pharmacy and Pharmacology·J P BaiJ G Buls
Jul 10, 2008·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Camile B WoitiskiFrancisco Veiga
May 24, 2005·International Journal of Pharmaceutics·Yoshinobu AokiKozo Takayama
Aug 24, 2001·The Journal of Pharmacy and Pharmacology·V AgarwalM A Khan
May 18, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Lucia SalvioniMiriam Colombo
Jan 16, 2018·Drug Discoveries & Therapeutics·Akihiro MatsumotoMasahiro Murakami

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