Immunohistochemical localization of NADPH-cytochrome P450 reductase in human tissues

Carcinogenesis
P M HallM E McManus

Abstract

In an attempt to elucidate the mechanism(s) behind the susceptibility of tissues to the toxic effects of chemicals, NADPH-cytochrome P450 reductase IgG has been used to map the distribution and localization of the cytochrome P450 system in hepatic and extrahepatic human tissues. Employing the Western blot procedure this antibody recognized a single band in human liver microsomes which corresponded in mol. wt to the purified reductase. Immunoreactive NADPH-cytochrome P450 reductase staining was detected in all zones of the liver acinus, with maximal staining in hepatocytes adjacent to the terminal hepatic venules (zone 3). Considerable variation in the intensity of reductase staining was observed in different segments of the gastrointestinal tract. Staining was most intense in the enterocytes of the small intestine, with maximal staining at the tips of the villi. Colonic epithelial cells were variably positive while the rectum was negative. Pancreatic ductal cells were positive whereas exocrine cells were negative. In the lung, reductase was detected in bronchiolar and bronchial epithelial cells, Clara cells and alveolar lining cells. In the kidney, the proximal and distal convoluted tubules, the loops of Henle, the collecting d...Continue Reading

Citations

Jan 14, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D S Keeney, M R Waterman
Jan 1, 1991·Journal of Biochemical Toxicology·C W Wheeler, T M Guenthner
Apr 24, 2004·Biochemical Pharmacology·Diane J Lees MurdockChristopher R Barnett
Jun 25, 1999·Free Radical Biology & Medicine·J Johnson InbarajR Murugesan
Mar 1, 1997·The International Journal of Biochemistry & Cell Biology·A Clouter, R J Richards
Aug 4, 2001·Biochemical and Biophysical Research Communications·D C LambS L Kelly
Jan 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Takaaki AkaikeHiroshi Maeda
May 28, 2010·Expert Opinion on Drug Metabolism & Toxicology·Shiuan-Woei LinWuFu-Chuo Peng
Jan 1, 1991·Critical Reviews in Toxicology·L S Kaminsky, M J Fasco
May 2, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Lynn Berndt-WeisCarole Yauk
Jan 22, 2008·Free Radical Biology & Medicine·Yun WangJeffrey D Laskin
Feb 27, 2007·Free Radical Biology & Medicine·Griselda R BorthiryCharles R Myers
Nov 29, 2005·Nitric Oxide : Biology and Chemistry·Teruo AkutaTakaaki Akaike
Jul 11, 2000·Japanese Journal of Pharmacology·T NakamuraY Yamazoe
Feb 19, 2008·Journal of Inorganic Biochemistry·Griselda R BorthiryCharles R Myers
Jun 7, 2005·Journal of Pharmacological Sciences·Mohammad Hasan ZakiTakaaki Akaike
Nov 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R A McKinnonM E McManus
Oct 1, 1992·Biopharmaceutics & Drug Disposition·R W SallieR Williams
Sep 2, 2004·British Journal of Cancer·T HigenbottamY Fujita
Mar 6, 2004·Pharmacological Reviews·Martijn RooseboomNico P E Vermeulen
Oct 26, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Shou-Lin WangJun-Yan Hong
Oct 11, 2011·Molecular Pharmacology·Christopher P GuiseAdam V Patterson
Mar 9, 2006·Alternatives to Laboratory Animals : ATLA·Sandra CoeckeAndrew Worth
Aug 10, 2000·Biochemical Pharmacology·T UenoS Kimura
Sep 10, 2021·NPJ Genomic Medicine·Mahboubeh R RostamiRonald G Crystal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.