PMID: 9450532Feb 5, 1998Paper

Immunohistochemical localization of nicotinic acetylcholine receptors in the guinea pig bowel and pancreas

The Journal of Comparative Neurology
A L Kirchgessner, M T Liu

Abstract

Although nicotinic acetylcholine receptors (nAChRs) are known to be present on neural elements in both the bowel and the pancreas, the precise location of these receptors has not previously been determined. Immunocytochemistry, by using a rat monoclonal antibody (mAb35), which recognizes alpha-bungarotoxin (alpha-Bgt)-insensitive nAChRs, and a polyclonal antibody raised against the alpha-Bgt-sensitive receptor subunit, alpha7, was used to locate receptor protein in guinea pig gut and pancreas. mAb35-receptor (mAb35-R) immunoreactivity was abundant in both enteric plexuses, enterochromaffin cells, and pancreatic ganglia. Immunostaining was associated with the cell membrane, and clusters of mAb35-R were observed on cell somas and dendrites. Receptor immunoreactivity was also observed on terminals and axons, suggesting that a subset of nAChRs is presynaptic. Internal sites of mAb35-R were observed in permeabilized ganglia. Cells expressing the receptors were closely associated with ChAT-immunoreactive nerve fibers. In addition, the majority of ChAT-positive neurons expressed both cell surface and internal stores of mAb35-R. In the bowel, clusters of mAb35-R were present on the soma and dendrites of Dogiel type I motorneurons and s...Continue Reading

Citations

Dec 5, 2002·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J J Galligan
Aug 4, 2011·Journal of Translational Medicine·Shaheen E Lakhan, Annette Kirchgessner
Apr 15, 1999·Annual Review of Neuroscience·A B MacDermottS A Siegelbaum
Nov 23, 2006·Autonomic Neuroscience : Basic & Clinical·Jeffrey A LoveTina G Smith
Jul 31, 2004·The Journal of Physiology·Leonid G ErmilovJoseph H Szurszewski
Apr 7, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J J Galligan, R A North
Feb 4, 2003·The Journal of Physiology·David R LindenGary M Mawe
Jun 6, 2003·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·L G ErmilovJ H Szurszewski
Dec 26, 2006·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·H L Cameron, M H Perdue
Sep 19, 2006·British Journal of Pharmacology·C L Hirota, D M McKay
Jan 13, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·F ReichardtM Schemann
Jan 3, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·L DurninV N Mutafova-Yambolieva

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