PMID: 6987197Feb 1, 1980Paper

Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparations

Histochemistry
M CostaE Solcia

Abstract

A method is described for the immunohistochemical localization of peptides in whole-mount preparations. Tissue is fixed as laminae with a picric acid/formaldehyde mixture and then dehydrated, cleared and rehydrated before exposure to antibodies. This procedure ensures adequate penetration of the antibody molecules without the need to freeze and thaw the tissue or to use detergents, preserves antigenicity and lowers non-specific background staining. The laminae are incubated with the primary antisera for 16 h at room temperature and, after washing, with a second, fluorescent tagged, antiserum. This can be followed by a peroxidase-anti-peroxidase localization of the second antiserum, which acts as a bridge. The method gives a precise and reproducible localization of immunoreactive peptides, with good penetration and low background even in thick preparation. Large areas can be scanned and neuroeffector relationships studied more easily than in sections.

References

Apr 30, 1979·Naunyn-Schmiedeberg's Archives of Pharmacology·R FrancoJ B Furness
Sep 5, 1978·Cell and Tissue Research·R BuffaE Solcia
Jan 1, 1971·Histochemie. Histochemistry. Histochimie·J B Furness, M Costa
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·B K HartmanS Udenfriend
Dec 31, 1973·Zeitschrift für Zellforschung und mikroskopische Anatomie·J BarryP Poulain
Oct 14, 1967·Nature·M StefaniniL Zamboni
Jan 1, 1971·Zeitschrift für Zellforschung und mikroskopische Anatomie·M Costa, G Gabella
Sep 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane
Nov 1, 1977·Neuroscience Letters·M CostaA Arimura

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Citations

Nov 1, 1994·Microscopy Research and Technique·T L PowleyJ B Kelly
Jan 1, 1983·Cell and Tissue Research·R E Papka, D H Matulionis
Jun 1, 1993·Cell and Tissue Research·I J Llewellyn-SmithJ C Bornstein
Jan 1, 1984·Archives of Oto-rhino-laryngology·R UddmanF Sundler
Jan 1, 1982·Archives of Oto-rhino-laryngology·R UddmanF Sundler
Nov 1, 1984·Naunyn-Schmiedeberg's Archives of Pharmacology·H TraurigF Lembeck
Jan 1, 1982·Histochemistry·K TornqvistB Ehinger
Jul 1, 1989·Fish Physiology and Biochemistry·K UematsuS Nilsson
Jul 15, 1987·Experientia·I J Llewellyn-Smith
Oct 1, 1987·Fish Physiology and Biochemistry·G Aldman, S Holmgren
Dec 4, 2004·Cell and Tissue Research·Masato NagahamaReiji Semba
Sep 13, 2006·Journal of Fluorescence·Parkash MandhanMichael J Sullivan
Oct 9, 1990·European Journal of Pharmacology·K I MaynardG Burnstock
Jun 1, 1987·General and Comparative Endocrinology·G S O'NeilM C Thorndyke

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