Immunohistochemical localization of prostaglandin H synthase in the epididymis and vas deferens of the mouse

Biology of Reproduction
P B MarshburnR B Shabanowitz

Abstract

Prostaglandins (PGE2, PGF2 alpha) in the excurrent ducts of the male reproductive tract appear to be both modulators of ductal contractility for transport of spermatozoa and factors involved in the regulation of sperm maturation. To identify the tissue sites for the production of prostaglandins (PGs) in the excurrent ductal system, we have employed an immunohistochemical technique to localize prostaglandin H (PGH) synthase in the epididymis and vas deferens of the mouse. A mouse monoclonal antibody to PGH synthase was used and was shown to be specific for the mouse enzyme by Western blot analysis. In sexually mature mice, PGH synthase was primarily localized to the epithelium of the epididymis and vas deferens. Within the epididymal epithelium, immunoactivity appeared in all cell types of the initial segment, in a subpopulation of cells with predominantly apically oriented nuclei in the caput and corpus, and in low levels in the cauda. PGH synthase reactivity was the most intense in the epithelial cells of the vas deferens. PGH synthase was not detected in smooth muscle cells, spermatozoa, or luminal fluid. This study suggests that the epithelium of the excurrent ductal system of the mouse is the major site for PG production. T...Continue Reading

Citations

May 1, 1993·Molecular Reproduction and Development·M A WinerD J Wolgemuth
May 8, 1998·Journal of Reproductive Immunology·M J LuckasD I Lewis-Jones
Jul 21, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·C BaumannF Theuring
Oct 30, 1992·Biochimica Et Biophysica Acta·S Yamamoto

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