Immunohistochemical localization of proteins in the nervous system

Current Protocols in Neuroscience
Laura A Volpicelli-Daley, Allan Levey

Abstract

The immunohistological methods described in this unit can be used to determine the precise localization of neurochemicals, receptors, and proteins throughout the nervous system. Determining the localization of a protein within defined brain nuclei and neuronal cell populations can provide important clues regarding its potential function. Immunoperoxidase reactions and light microscopy are commonly used to visualize the distribution of a single primary antibody directed to an antigen of interest. Double-labeling immunofluorescence and confocal microscopy techniques detect the localization of one protein relative to another protein and allow analysis of colocalization at a cellular and subcellular level. The colocalization of two proteins can also be quantified, allowing analysis of the extent of overlap between two labeled markers and measurements of changes in the localization of one protein relative to another following drug treatment or in animals that have been genetically modified. The theoretical limit of resolution of confocal microscopy is 0.1 to 0.2 microm.

References

Jun 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J C Adams
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A I LeveyB J Ciliax
May 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S A SchnellM W Wessendorf
Jun 5, 2003·Annals of Neurology·Laura A Volpicelli-DaleyAllan I Levey
Dec 4, 2003·Molecular Pharmacology·Laura A Volpicelli-DaleyAllan I Levey

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Citations

Nov 23, 2019·Current Protocols in Neuroscience·Ronald F Paletzki, Charles R Gerfen
Sep 15, 2007·The Journal of Clinical Investigation·Jennifer J MardenJohn F Engelhardt
May 31, 2019·The Journal of Biological Chemistry·Jessica M FroulaLaura A Volpicelli-Daley
Jun 27, 2018·Current Protocols in Neuroscience·Alexander D JacobSheena A Josselyn

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