PMID: 6172462Nov 1, 1981Paper

Immunohistochemical localization of retrogradely and anterogradely transported wheat germ agglutinin (WGA) within the central nervous system of the rat: application to immunostaining of a second antigen within the same neuron

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
R M LechanS Jacobson

Abstract

Immunohistochemical localization of retrogradely transported wheat germ agglutinin (WGA) is proposed as a sensitive histochemical technique to identify point to point connections within regions of the central nervous system. Injections of WGA into the median eminence of the hypothalamus and the caudate-putamen complex, respectively, were performed to illustrate that this material is rapidly transported over long distances and accumulates within the cytoplasm of neuronal perikarya and their processes. The applicability of this technique to identification of a second antigen within immunoreactive-WGA-labeled neurons is also demonstrated by sequential immunostaining of tyrosine hydroxylase within dopamine-containing cells of the mesencephalon, ipsilateral to an injection in the caudate-putamen complex. This technique is of use in characterizing bioaminergic neurons in the central nervous system and may also be of use in characterizing peptidergic neurons.

Citations

Jun 20, 1983·Brain Research·R G WileyD J Reis
Jul 1, 1983·Journal of Biochemical and Biophysical Methods·A G FowlerM H Koch
Apr 11, 1983·Neuroscience Letters·B S PhippsW E Foote
Feb 21, 1983·Neuroscience Letters·H Schnyder, H Künzle
Apr 16, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Kressel
Feb 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M A ArbabN A Svendgaard
Apr 15, 1986·The Journal of Comparative Neurology·R B Simerly, L W Swanson
May 1, 1988·The Journal of Comparative Neurology·G J De VriesP Yahr
Oct 1, 1993·The Journal of Laryngology and Otology·Y TanakaM Hirano
Nov 1, 1987·Journal of Neuroscience Methods·H TomimotoH Kimura

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