Immunohistochemical localization of the neural cell adhesion molecule in the rat sciatic nerve

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
S D DolapchievaW Kühnel

Abstract

The neural cell adhesion molecule (NCAM) has been widely studied in the early embryonal development of the nervous system. The data about NCAM distribution in the peripheral nerves during postnatal life are scant and some controversial. In the present study, the NCAM localization in the sciatic nerves of 15-day-old Wistar rats has been studied. Semi-thin sections of the nerves were immunotested with a polyclonal antibody (Santa Cruz Biotechnology) that recognizes rat NCAM. The antibody was visualized with donkey anti-goat IgG, conjugated to 12 nm colloidal gold, and silver amplification. In the myelinated nerve fibres, the immunoreactivity was associated with the axons, mainly with their plasma membrane, which was unstained in the nodes of Ranvier. The myelin sheaths and the myelinating Schwann cells were negative. The extracellular matrix and the bundles of non-myelinated nerve fibres were immunopositive.

References

Dec 3, 1990·Brain Research·S Bhat, D H Silberberg
Aug 1, 1986·The Journal of Cell Biology·F RiegerG M Edelman
Sep 1, 1986·The Journal of Cell Biology·J K DaniloffG M Edelman
Apr 1, 1986·Developmental Biology·K W TosneyU Rutishauser
Feb 4, 1983·Science·G M Edelman
Dec 1, 1993·Neuropathology and Applied Neurobiology·N Le ForestierR K Gherardi
Oct 1, 1994·Journal of Neurocytology·B Langenfeld-OsterA Wernig
Oct 22, 1993·The Journal of Comparative Neurology·D V SmithM T Shipley
Jan 1, 1997·Molecular and Cellular Neurosciences·H CremerA Represa
Dec 31, 1997·Journal of the Neurological Sciences·P H RocheJ F Pellissier

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Citations

Apr 9, 2002·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·S DolapchievaW Kühnel
Mar 23, 2006·Annals of Surgery·Hiram C PolkBruce Barraclough
Jul 12, 2011·Trends in Neurosciences·Peter K Stys

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