Immunohistochemical localization of the NH(2)-terminal and COOH-terminal fragments of dentin sialoprotein in mouse teeth.

Cell and Tissue Research
Guohua YuanShuo Chen

Abstract

Dentin sialoprotein (DSP) is a major non-collagenous protein in dentin. Mutation studies in human, along with gene knockout and transgenic experiments in mice, have confirmed the critical role of DSP for dentin formation. Our previous study reported that DSP is processed into fragments in mouse odontoblast-like cells. In order to gain insights into the function of DSP fragments, we further evaluated the expression pattern of DSP in the mouse odontoblast-like cells using immunohistochemistry and western blot assay with antibodies against the NH(2)-terminal and COOH-terminal regions of DSP. Then, the distribution profiles of the DSP NH(2)-terminal and COOH-terminal fragments and osteopontin (OPN) were investigated in mouse teeth at different ages by immunohistochemistry. In the odontoblast-like cells, multiple low molecular weight DSP fragments were detected, suggesting that part of the DSP protein was processed in the odontoblast-like cells. In mouse first lower molars, immunoreactions for anti-DSP-NH(2) antibody were intense in the predentin matrix but weak in mineralized dentin; in contrast, for anti-DSP-COOH antibody, strong immunoreactions were found in mineralized dentin, in particular dentinal tubules but weak in predentin...Continue Reading

References

Nov 1, 1992·Matrix : Collagen and Related Research·W T ButlerR A Foster
Mar 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R N D'SouzaW T Butler
Jan 1, 1995·Connective Tissue Research·H H RitchieW T Butler
Apr 16, 1998·European Journal of Oral Sciences·W T Butler
Jun 18, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·F BleicherH Magloire
Oct 6, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J SodekM D McKee
Apr 6, 2001·Calcified Tissue International·A BoskeyW T Butler
Jun 20, 2002·Calcified Tissue International·A L BoskeyM D McKee
Jul 5, 2002·Journal of Dental Research·C QinW T Butler
Dec 20, 2002·Connective Tissue Research·A UnterbrinkM MacDougall
Jun 6, 2003·European Journal of Oral Sciences·Chunlin QinWilliam T Butler
Sep 4, 2003·Connective Tissue Research·Larry W Fisher, Neal S Fedarko
Sep 4, 2003·Connective Tissue Research·Chunlin QinWilliam T Butler
Feb 6, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Michel Goldberg, Anthony J. Smith
Feb 11, 2004·The Journal of Biological Chemistry·Philippe H TartaixAdele L Boskey
Apr 2, 2004·European Journal of Oral Sciences·Otto BabaWilliam T Butler
Jun 10, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C QinW T Butler
Aug 27, 2004·Journal of Dental Research·K U E Ogbureke, L W Fisher
Nov 10, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Otto BabaWilliam T Butler
Nov 13, 2004·The Journal of Biological Chemistry·Yasuo YamakoshiJames P Simmer
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Larry W FisherNeal S Fedarko
Jun 28, 2005·The Journal of Biological Chemistry·Shuo ChenMary Macdougall
Jul 15, 2005·The Journal of Biological Chemistry·Michael L PaineMalcolm L Snead
Oct 19, 2006·The Journal of Biological Chemistry·Yasuo YamakoshiJames P Simmer
Oct 21, 2006·The Journal of Biological Chemistry·Sivakumar GajjeramanAnne George
Dec 26, 2006·The Journal of Biological Chemistry·Shane N WhiteMalcolm L Snead
Apr 25, 2007·Journal of Dental Research·J-W Kim, J P Simmer
Oct 16, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Izabela MaciejewskaChunlin Qin
Nov 13, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jianjun HaoAnne George
Apr 8, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Shigeki SuzukiAshok B Kulkarni
Feb 17, 2010·Journal of Oral Biosciences·Yasuo Yamakoshi
Mar 25, 2010·Journal of Dental Research·Y SunC Qin
Apr 20, 2010·Journal of Dental Research·Q ZhuC Qin

❮ Previous
Next ❯

Citations

May 3, 2014·Nanomedicine·Hye Sung Kim, Hyuk Sang Yoo
Jul 17, 2020·Frontiers in Physiology·Xiaohui GouGuohua Yuan
Apr 20, 2018·International Journal of Cell Biology·Paula A BaldiónJaime E Castellanos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.