Immunohistochemical localization of the epithelial sodium channel in the rat inner ear

Hearing Research
Shi-Xun Zhong, Zhao-Hua Liu

Abstract

Endolymph in membranous labyrinth is a K+-rich and Na+-poor fluid, and perilymph is conversely Na+-rich and K+-poor. Electrolyte transport between endolymph and perilymph is important for regulation of volume and osmotic pressure of the labyrinth. The epithelial sodium channel (ENaC) is a good candidate protein for Na+ transport in the tight epithelia, which has been well demonstrated in other tissues such as kidney, colon and lung. The purpose of the present study was to investigate the cellular localization of ENaC subunits in the rat inner ear immunohistochemically with the specific polyclonal rabbit antibodies against the rat alpha-, beta- and gamma-ENaC. All three subunits of ENaC were extensively labeled in the cochlea including the stria vascularis, spiral ligament, organ of Corti, spiral limbus, Reissner's membrane and spiral ganglion, and in the vestibule including the sensory epithelia and stroma cells of the macula utriculi, macula sacculi and ampullary crest. In conclusion, our results suggest that functional ENaC in the labyrinth may work in concert with other Na+ and K+ transport molecules to regulate endolymph and to maintain homeostasis in the inner ear.

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Citations

Sep 9, 2008·Pflügers Archiv : European journal of physiology·Zoltán Rusznák, Géza Szucs
Dec 21, 2012·Human Molecular Genetics·Laurence MolinaBenjamin Delprat
May 31, 2011·Hearing Research·Sung Huhn Kim, Daniel C Marcus
Oct 21, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Poornapriya RamamurthyKate F Barald
Jun 30, 2018·Physiological Genomics·Janet L Fitzakerley, George J Trachte
Sep 6, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Md Shahrier AminFrans H H Leenen
Aug 3, 2007·American Journal of Physiology. Cell Physiology·Florian LangPhiline Wangemann
Mar 21, 2009·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Sung Huhn KimJae Young Choi
Dec 4, 2008·Acta Oto-laryngologica·Shi-Xun Zhong, Zhao-Hua Liu

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