Immunohistochemical localization of the catecholamine-synthesizing enzymes, substance P and enkephalin in the human fetal sympathetic ganglion

Cell and Tissue Research
A HervonenR J Miller

Abstract

The human fetal sympathetic ganglia were studied using the indirect peroxidase-antiperoxidase PAP method for immunocytochemical demonstration of three catecholamine-synthesizing enzymes, tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine-N-methyltransferase (PNMT) as well as the neuropeptides leucine (Leu5)-enkephalin and substance P. The neuroblasts of the ganglia showed intense peroxidase immunoreactivity for TH, moderate reaction to DBH, and no reaction to PNMT. The small intensely fluorescent (SIF) cells situated along the blood vessels also showed positive labelling for only two enzymes, TH and BDH. The immunocytochemical localization of these enzymes suggests that both neuroblasts and SIF cells synthesize noradrenalin. Neither the neuroblasts nor SIF cells showed a reaction to substance P, and only the SIF cells contained enkephalin-like immunoreactivity. The role of enkephalin in the noradrenalin-containing SIF cells is unknown, but may be related to neuromodulation of ganglionic transmission.

Citations

Jan 1, 1987·Archives of Gynecology·A SchoenfeldJ Ovadia
Aug 1, 1988·Mechanisms of Ageing and Development·R JärviA Hervonen
Sep 12, 1996·Journal of the Autonomic Nervous System·G SimonettaI C McMillen
Nov 1, 1982·Peptides·G A OlsonD H Coy
Jan 1, 1984·Neurobiology of Aging·M PartanenS I Rapoport
Apr 1, 1993·Biological Psychology·J A McCubbin
Jan 1, 1983·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P Helén, A Hervonen
Dec 22, 1999·Pathology, Research and Practice·K Kawabata
Aug 1, 1989·Journal of Electron Microscopy Technique·A HervonenM Pelto-Huikko
Jul 1, 1988·The Journal of Urology·R CroweJ K Light
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