Immunohistochemical localization of troponin-C in cultured neurons

Journal of Muscle Research and Cell Motility
F J RoisenT Hirabayashi

Abstract

Our previous immunofluorescence studies on neurons have demonstrated the presence of myosin in regions of neurons which contained actin. To determine if a system similar to the troponin complex of striated muscle is present in neurons, antibody shown to be specific for the calcium-binding component of troponin (troponin-C) was applied to cultures of embryonic chick and rat dorsal root ganglia. Neurites treated with anti-troponin-C exhibited a bright fluorescence. Accompanying non-neuronal cells were less reactive than the neuronal elements. Immunodiffusion and immunofluorescence showed that the anti-troponin-C did not react with calmodulin, whereas homogenates of the ganglia elicited a positive immunochemical reaction with the anti-troponin-C in Ouchterlony tests. Our results suggest that some intra-axonal movements may be generated by the interaction of actin and myosin and controlled in part by a calcium-troponin-C-dependent mechanism.

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Citations

Sep 8, 1995·The Journal of Biological Chemistry·A N Akopian, J N Wood
Nov 6, 2007·American Journal of Physiology. Cell Physiology·Michael Henrich, Keith J Buckler
Apr 11, 2014·BioMed Research International·Oksana S BychenkoEugene D Sverdlov

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