Immunohistochemical localization of type 1 11beta-hydroxysteroid dehydrogenase in human tissues

The Journal of Clinical Endocrinology and Metabolism
M L RickettsP M Stewart

Abstract

Two isozymes of 11beta-hydroxysteroid dehydrogenase (11betaHSD) catalyze the interconversion of hormonally active cortisol to inactive cortisone. Activity and messenger ribonucleic acid studies indicate that type 1 11betaHSD (11betaHSD1) is expressed in glucocorticoid target tissues such as liver, gonad, and cerebellum, where it regulates the exposure of cortisol to glucocorticoid receptors. To further understand the role of 11betaHSD1 in human tissues, we have studied the localization of this isozyme using an antibody raised in sheep against amino acids 19-33 of human 11betaHSD1. Western blot analyses indicated that the immunopurified antibody recognized a band of approximately 34 kDa in human liver and decidua. Immunoperoxidase studies on liver, adrenal, ovary, decidua, and adipose tissue indicated positive cytoplasmic staining for 11betaHSD1. 11BetaHSD1 immunoreactivity was observed more intensely around the hepatic central vein, with no staining around the portal vein, hepatic artery, or bile ducts. No staining for 11betaHSD1 was observed in the adrenal medulla, but 11betaHSD1-immunoreactive protein was observed in all three zones of the adrenal cortex, with the most intense staining in the zona reticularis > zona glomerulo...Continue Reading

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Citations

Oct 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Li LiuS Stanley Young
Apr 19, 2008·Annals of the Rheumatic Diseases·R HardyM S Cooper
Feb 18, 2010·American Journal of Physiology. Endocrinology and Metabolism·Dale S EdgertonAlan D Cherrington
Dec 31, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Giulia Chinetti-GbaguidiBart Staels
Nov 19, 2010·Lipids in Health and Disease·Sakamuri S S Vara PrasadAyyalasomayajula Vajreswari
Nov 28, 2007·Endocrinology·Elise P Gomez-SanchezCelso E Gomez-Sanchez
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Aug 7, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·R Nass, M O Thorner
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