Immunohistochemical localization of type I and II collagens in the involuting chick notochords in vivo and in vitro

Cell Biology International
E Ghanem

Abstract

Embryonic chick notochords were studied during their metabolically active and involuting periods for the expression of collagen type I and II. The staining was carried out on notochords in vivo at stage 20 and stage 35 and on mesenchyme-contaminated and mesenchyme-free notochords at stage 20, which were cultured in vitro for 6 days. The results show that type II collagen is demonstrable in the notochords, at all the examined stages, both in vivo and in vitro. However, the expression of type I collagen was stage-dependent in vivo and in vitro. At stage 20, the perinotochordal sheath is positively immunostained for collagen type I, but the notochord itself is negative. At stage 35, the perinotochordal sheath as well as the notochord are positively immunostained for collagen type I. The mesenchyme-contaminated and the mesenchyme-free notochords and their sheaths are also positively immunostained for the type I collagen after 6 days in vitro. The current results, at late developmental stages, indicate that the involuting notochords express collagen type I, which seems not to be altered by changing the micro-environment in vivo.

Citations

Nov 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Guangjun Zhang, Martin J Cohn
Jul 12, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Joan CerdàMichael Brand
Oct 25, 2016·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Charlotte GistelinckPaul J Coucke

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