Immunohistochemical staining of bromodeoxyuridine-labelled cells in the mouse embryo.

Journal of Microscopy
D C CottellJ G Bannigan

Abstract

The aim of this study was to compare two immunohistochemical methods, avidin-biotin peroxidase and immunogold silver staining (IGSS), in the detection of 5-bromodeoxyuridine incorporation in mouse embryo tissues. In addition, two fixation schedules, formal-saline and a mixture of formaldehyde and glutaraldehyde (4FIG), and two embedding procedures, paraffin wax and the acrylic resin L.R. White, were also compared. Pregnant mice were injected with 600 mg per kilogram body weight on days 10 or 15 (plug day = day 1) of gestation and the embryos recovered 2 h after treatment and fixed in formal-saline or 4FIG. Fixed material was then processed into wax blocks or L. R. White resin. After sectioning, the antigen-antibody reaction was visualized using either the avidin-biotin peroxidase or IGSS methods. All methods tested gave a well-contrasted, highly specific reaction, but IGSS in combination with 4FIG and L. R. White gave a clearer and more sensitive signal than other combinations.

References

Aug 18, 1975·Annals of the New York Academy of Sciences·R G Skalko, D S Packard
Nov 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B SchutteG H Blijham
Jan 1, 1984·Histochemistry·D R SpringallJ M Polak
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Jul 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C S HolgateC C Bird
Nov 1, 1983·The Journal of Clinical Investigation·G MorstynJ B Mitchell
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F DolbeareJ W Gray
Jan 1, 1981·Anatomy and Embryology·J BanniganA van Breda
Feb 1, 1963·The Journal of Comparative Neurology·S FUJITA

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Citations

Aug 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M GalottoR Cancedda
Jul 5, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Laura B NgwenyaDouglas L Rosene
Jun 9, 1997·Biochemical and Biophysical Research Communications·S MitsuyoshiN Sakaguchi

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