PMID: 2499621Jul 1, 1989Paper

Immunohistochemical study of arachidonate 12-lipoxygenase in porcine tissues

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
T MaruyamaK Watanabe

Abstract

12-Lipoxygenase oxygenates the 12 position of arachidonic acid and produces its 12-hydroperoxy derivative. The enzyme is found in greatest amounts in porcine leukocytes and is distributed widely in various other tissues. An anti-12-lipoxygenase antibody was raised in rabbits with the immunoaffinity-purified enzyme as an antigen and was used in immunohisto- and cytochemical studies on the enzyme, the physiological significance of which remains to be clarified. When peripheral blood cells were examined by immunoelectron microscopy, the enzyme was found in neutrophils and monocytes but was not detected in lymphocytes, platelets, and erythrocytes. In immunostained neutrophils and monocytes the enzyme was localized in the cytosol but was not clearly detected in the plasma membrane, nuclear membrane, endoplasmic reticulum, and other organelles. Several other organs known to contain considerable amounts of 12-lipoxygenase were also investigated immunohistochemically, i.e., alimentary tract (ileum and jejunum), lymphatic organs (spleen, lymph node, and thymus), ovary, lung, liver, and others. In these organs, resident mast cells and granulocytes infiltrating the interstitial tissues were positively immunostained. The enzyme was not det...Continue Reading

Citations

Sep 11, 1991·Biochimica Et Biophysica Acta·K NatsuiK Watanabe
Oct 30, 1992·Biochimica Et Biophysica Acta·S Yamamoto
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·T Yoshimoto, S Yamamoto
Jan 16, 2002·Prostaglandins & Other Lipid Mediators·Hiroaki TaniguchiKiyoshi Okuda
Mar 1, 1997·Progress in Lipid Research·S YamamotoN Ueda
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C D FunkB Samuelsson
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R NatarajanJ Nadler
Apr 1, 1993·The American Review of Respiratory Disease·V R ShannonM J Holtzman
Jan 2, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Shiro KurusuMitsumori Kawaminami
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·M J CoffeyV B O'Donnell
Sep 21, 2005·Biochemical and Biophysical Research Communications·Shozo YamamotoNatsuo Ueda

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