PMID: 11897810Mar 19, 2002Paper

Immunohistochemical study of osteopontin expression during distraction osteogenesis in the rat

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Daniel S PerrienCharles K Lumpkin

Abstract

Distraction osteogenesis (DO) is a limb-lengthening procedure that combines mechanical tension stress with fracture healing to provide a unique opportunity for detailed histological examination of bone formation. Osteopontin (OPN) is a multifunctional matricellular protein believed to play a key role in wound healing and cellular response to mechanical stress. We studied the expression of OPN during DO using standard immunohistochemical (IHC) staining techniques. In addition, we compared the expression of OPN to proliferation (PCNA-positive cells) in the DO gap. After 14 days of distraction in the rat, these stains revealed variations in OPN expression and its relationship to proliferation according to the cell type, tissue type, and mode of ossification examined. Fibroblast-like cells within the central fibrous area exhibited intermittent low levels of OPN, but no relationship was observed between OPN and proliferation. In areas of transchondral ossification, OPN expression was very high in the morphologically intermediate oval cells. During intramembranous ossification, osteoblasts appeared to exhibit a bimodal expression of OPN. Specifically, proliferating pre-osteoblasts expressed osteopontin, but OPN was not detected in th...Continue Reading

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Citations

Aug 15, 2009·Tissue Engineering. Part a·Jasmin LienauHanna Schell
Jun 16, 2012·Journal of Biomedicine & Biotechnology·Joana ReisJosé Potes
Feb 26, 2016·Journal of the Mechanical Behavior of Biomedical Materials·Susmita MukherjeePradip Kumar Das
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