Immunohistochemical study of seminiferous epithelium in adult bovine testis using monoclonal antibodies against Ki-67 protein and proliferating cell nuclear antigen (PCNA)

Cell and Tissue Research
K H WrobelR Kujat

Abstract

The distribution pattern of proliferating cell nuclear antigen (PCNA) and Ki-67 protein was studied in adult bovine seminiferous epithelium by means of immunohistochemistry using monoclonal antibodies. Tailoring the methodological protocol for each of the two proliferation markers was a necessary prerequisite for obtaining optimal results in tubular sections and whole-mounts. A-, I- and B-spermatogonia displayed PCNA-positive nuclei, except during meta-, ana- and telophases of mitosis. PCNA-negative nuclei in the basal tubular compartment, excluding those from non-cycling Sertoli cells, belonged to the spermatogonia precursor cell line. However, only about 30%, 45% and 47% of the respective A-, I-, B-spermatogonia had positive nuclei after exposure to the MIB-1 antibody directed against the Ki-67 protein. Spermatogonia with MIB-1-negative nuclei represented cells in the G1-phase. Both antibodies reacted intensely with the nuclei of preleptotene primary spermatocytes. PCNA reactivity was also present during leptotene through pachytene. Ki-67 protein expression was absent during leptotene and zygotene but was again encountered during pachytene and meiosis I and II. Anti-PCNA/anti-protein gene product 9.5 double-labelling indicate...Continue Reading

Citations

Mar 29, 2000·Molecular Reproduction and Development·N N AtanassovaC A Valkova
Dec 18, 2001·The Anatomical Record·T UenoT Yamamoto
May 25, 2004·Nature Genetics·José A CostoyaPier Paolo Pandolfi
Sep 30, 2000·International Journal of Andrology·M R MillarP T Saunders
Aug 31, 1999·Development, Growth & Differentiation·C MiuraK Yamauchi
Aug 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lea A MedeirosRudolf Jaenisch
Dec 1, 2005·Development, Growth & Differentiation·Taketsugu FujibuchiKatsumi Kito
Oct 6, 2007·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Gunseli GuvenZafer C Cehreli
Nov 11, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Rachel E DickinsonW Colin Duncan
Nov 20, 2008·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M R D'AndreaB P Damiano
Oct 8, 2005·Molecular Reproduction and Development·Kenichiro HataHiroyuki Sasaki
Feb 12, 2010·Biology of Reproduction·Byung-Gak KimBuom-Yong Ryu
May 21, 2013·Reproduction, Fertility, and Development·Yi ZhengWenxian Zeng
Oct 8, 1999·Journal of Dental Research·E T KobayashiH Sakai
Aug 20, 2011·Journal of Cell Science·Annegret BördleinAndreas Winterpacht

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