PMID: 9543338May 30, 1998Paper

Immunohistochemistry and in situ hybridization of the androgen receptor in the developing human prostate

Anatomy and Embryology
G AumüllerG Verhoeven

Abstract

As it is suggested that the androgen receptor mechanism is required for prostatic development, we attempted to determine the appearance, expression and distribution of the androgen receptor in embryonic, infantile and pubertal human prostate. Using mono- and polyclonal antibodies and a digoxigenin-labeled 713 bp riboprobe, the androgen receptor expression in paraffin sections of fetal, infantile, and pubertal prostates was studied at the protein and RNA level. Under highly standardized conditions, application of the polyclonal antibodies resulted in a weak cytoplasmic and nuclear labeling of the epithelium of fetal glands. No immunoreaction was obtained with monoclonal antibodies. Applying the polyclonal antibody to pubertal and adult specimens, immunoreactivity of the androgen receptor was positive in nuclei of adluminal and basal epithelial cells, in interstitial and vascular smooth muscle cells and vascular endothelium, whereas ganglionic cells and enteroendocrine cells were negative. In situ hybridization with the digoxigenin-labeled riboprobe gave clear positive results already in epithelium of very young fetal specimens. A semiquantitative visual evaluation of in situ hybridizations showed that intermediate intensity of e...Continue Reading

Citations

May 1, 1999·Urology·G AumüllerP A Abrahamsson
Jan 16, 1999·Baillière's Clinical Endocrinology and Metabolism·O HiortE M Nitsche
Feb 2, 2000·The EMBO Journal·J M MüllerR Schüle
Aug 31, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Yasmin SajjadGill Vince
Sep 17, 2013·The Prostate·Camelia M SaffariniKim Boekelheide
Jan 4, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Evi DenoletGuido Verhoeven
Jun 15, 2010·Best Practice & Research. Clinical Endocrinology & Metabolism·Ralf WernerOlaf Hiort
Nov 1, 2000·The Prostate. Supplement·C S Foster
Jan 24, 2007·Current Urology Reports·Rosalia Misseri
Jan 14, 2000·Neonatal Network : NN·C LundD A Raines
Nov 19, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Saravana Mohan DhanasekaranArul M Chinnaiyan
Apr 25, 2007·Ultrastructural Pathology·Gianandrea PasquinelliEnrico Lucarelli
Feb 9, 2008·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Y I Goh, G Koren
Feb 19, 2000·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·R Spencer
Mar 23, 2018·BMJ Case Reports·Dominika M ZoltowskaJagadeesh K Kalavakunta

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