Immunohistochemistry and real-time PCR as diagnostic tools for detection of Borrelia burgdorferi sensu lato in ticks collected from humans

Experimental & Applied Acarology
Violeta T BriciuIngrid Huber

Abstract

The objective of this study was to evaluate different methods used for detection of Borrelia burgdorferi sensu lato (s.l.) in ticks: immunohistochemistry followed by focus floating microscopy (FFM) and real-time polymerase chain reaction (real-time PCR) targeting the ospA and hbb genes. Additionally, an optimized ospA real-time PCR assay was developed with an integrated internal amplification control (IAC) for the detection of inhibition in the PCR assay and was validated as an improved screening tool for B. burgdorferi. One hundred and thirty-six ticks collected from humans in a hospital from Cluj-Napoca, Romania, were investigated regarding genus, stage of development and sex, and then tested by all three assays. A poor quality of agreement was found between FFM and each of the two real-time PCR assays, as assessed by concordance analysis (Cohen's kappa), whereas the agreement between the two real-time PCR assays was moderate. The present study argues for a low sensitivity of FFM and underlines that discordant results of different assays used for detection of B. burgdorferi in ticks are frequent.

References

Feb 1, 1991·American Journal of Clinical Pathology·C E GreerM M Manos
Dec 1, 1986·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·V Preac-MursicG Schierz
Oct 1, 1995·The American Journal of Tropical Medicine and Hygiene·A M De Silva, E Fikrig
Jun 1, 1994·PCR Methods and Applications·C E GreerM M Manos
May 1, 1998·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·O KahlG Stanek
Jun 5, 2003·Emerging Infectious Diseases·Dania RichterFranz-Rainer Matuschka
Dec 10, 2003·Journal of Clinical Microbiology·Jeffrey HoorfarPatrick Fach
Mar 9, 2004·Journal of Clinical Microbiology·M Collares-PereiraC Cunha
Jul 16, 2005·Clinical Microbiology Reviews·Maria E Aguero-RosenfeldGary P Wormser
Sep 23, 2006·Diagnostic Microbiology and Infectious Disease·Lynn IvacicNader Ghebranious
Nov 24, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Gwenn GaumondSandra Bushmich
Jan 11, 2007·American Journal of Clinical Pathology·Klaus EisendleBernhard Zelger
Jun 19, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·T CerarF Strle
May 11, 2010·Journal of Microbiological Methods·Jana FerdinEva Ruzić-Sabljić
Mar 19, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·G Stanek, M Reiter
Sep 10, 2011·Lancet·Gerold StanekFranc Strle

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Citations

Mar 18, 2016·PLoS Neglected Tropical Diseases·Sara MoutaillerMuriel Vayssier-Taussat
Sep 26, 2019·Parasites & Vectors·Zsuzsa KalmárAndrei Daniel Mihalca
May 23, 2020·Pathogens·Zsuzsa KalmárAndrei Daniel Mihalca

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