PMID: 9524195May 9, 1998Paper

Immunohistochemistry of carbonic anhydrase isozyme IX (MN/CA IX) in human gut reveals polarized expression in the epithelial cells with the highest proliferative capacity

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
J SaarnioWilliam S Sly

Abstract

MN/CA IX is a recently discovered member of the carbonic anhydrase (CA) gene family that has been identified in the plasma membranes of certain tumor and epithelial cells and found to promote cell proliferation when transfected into NIH3T3 cells. This study presents localization of MN/CA IX in human gut and compares its distribution to those of CA I, II, and IV, which are known to be expressed in the intestinal epithelium. The specificity of the monoclonal antibody for MN/CA IX was confirmed by Western blots and immunostaining of COS-7 cells transfected with MN/CA IX cDNA. Immunohistochemical stainings of human gut revealed prominent polarized staining for MN/CA IX in the basolateral surfaces of the enterocytes of duodenum and jejunum, the reaction being most intense in the crypts. A moderate reaction was also seen in the crypts of ileal mucosa, whereas the staining became generally weaker in the large intestine. The results indicate isozyme-specific regulation of MN/CA IX expression along the cranial-caudal axis of the human gut and place the protein at the sites of rapid cell proliferation. The unique localization of MN/CA IX on the basolateral surfaces of proliferating crypt enterocytes suggests that it might serve as a liga...Continue Reading

References

Apr 1, 1992·Virology·S PastorekováJ Závada
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·N X Krueger, H Saito
Sep 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J I GordonK A Roth
Jul 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S ParkkilaH Rajaniemi
Jan 1, 1985·Annual Review of Physiology·M Lipkin
Mar 11, 1983·Nucleic Acids Research·H Luthman, G Magnusson
Jan 1, 1995·Annual Review of Biochemistry·W S Sly, P Y Hu
Oct 14, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·A K ParkkilaH Rajaniemi
Oct 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T JuvonenH Rajaniemi
May 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S ParkkilaH Rajaniemi
May 8, 1993·International Journal of Cancer. Journal International Du Cancer·J ZávadaV Zelník
Dec 1, 1995·The Histochemical Journal·A K ParkkilaH Rajaniemi
Nov 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S ParkkilaH Rajaniemi

❮ Previous
Next ❯

Citations

Oct 22, 2003·Experimental Cell Research·Eliska SvastováSilvia Pastoreková
Apr 2, 2011·The Journal of Biological Chemistry·Ying LiSusan C Frost
Mar 10, 2012·American Journal of Physiology. Cell Physiology·Jinghua LiuLane L Clarke
May 25, 2006·BMC Developmental Biology·Heini KallioSeppo Parkkila
Mar 18, 2009·BMC Developmental Biology·Shu-Yuan LiaoEric J Stanbridge
Mar 17, 2007·BMC Molecular Biology·Pei-wen PanSeppo Parkkila
Oct 19, 2004·Reproductive Biology and Endocrinology : RB&E·Piritta HynninenSeppo Parkkila
Oct 11, 2005·Neuro-oncology·Martin A ProescholdtMarsha J Merrill
Sep 30, 2006·The Keio Journal of Medicine·Jonathan D Kaunitz, Yasutada Akiba
Oct 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mari LeppilampiMarkus Sjöblom
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·S V IvanovM I Lerman
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·B UlmasovD N Silverman
Jan 1, 2013·Okajimas Folia Anatomica Japonica·Shinichi AbeBaik Hwan Cho
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·O TüreciW S Sly
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·A WaheedW S Sly
Dec 31, 2014·International Journal of Molecular Sciences·Huei Leng Helena NgZhijun Yang
Mar 5, 2016·Disease Markers·Ursa Brown-GlabermanAlison T Stopeck
Mar 25, 2006·The Journal of Physiology·Misa MizumoriYasutada Akiba
Jan 11, 2007·Alimentary Pharmacology & Therapeutics·J D Kaunitz, Y Akiba
Jan 11, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Piritta HynninenEija Tomas
Mar 26, 2013·International Journal of Urology : Official Journal of the Japanese Urological Association·Kazuhiro Yoshimura, Hirotsugu Uemura
Jul 3, 2013·Acta histochemica·Martina FurjelováMarian Adamkov
Aug 4, 2015·Future Oncology·Fouad AounThierry Roumeguère
May 27, 2015·Clinical Genitourinary Cancer·Avelino FragaRui Medeiros
Nov 27, 2010·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·G SergeantB Topal
Apr 20, 2016·Journal of Biomolecular Structure & Dynamics·Amresh PrakashMd Imtaiyaz Hassan
Mar 10, 2001·The American Journal of Pathology·S IvanovE J Stanbridge
Apr 28, 2005·International Journal of Cancer. Journal International Du Cancer·Magdalena KowalewskaJanusz A Siedlecki
Dec 2, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S ParkkilaH Rajaniemi
Jan 5, 2002·The Annals of Otology, Rhinology, and Laryngology·S E AxfordJ A Koufman
Oct 11, 2003·Alimentary Pharmacology & Therapeutics·M JuhászM P A Ebert
Mar 22, 2018·Cancer Biology & Therapy·Zhijuan ChenSusan C Frost
Jan 6, 2005·World Journal of Gastroenterology : WJG·Antti-J KiveläSeppo Parkkila

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.