Immunohistochemistry of opioid peptides in the guinea pig endocrine pancreas

Cell and Tissue Research
Y Cetin


Previous immunochemical investigations have demonstrated various opioid peptides in the pancreas. However, controversies exist related to the cellular localization of these peptides in the endocrine pancreas. Therefore, the guinea pig endocrine pancreas was immunohistochemically investigated for the presence of opioid peptides derived from pro-dynorphin, pro-enkephalin or pro-opiomelano-cortin. Immunoreactivities were demonstrated on serial semithin sections by the peroxidase anti-peroxidase technique. In routinely immunostained sections, immunoreactivities for dynorphin A and alpha-neo-endorphin were localized in pancreatic enterochromaffin cells, but not in islet cells. Immunoreactivity for Met-enkephalin was confined exclusively to B-cells and was localized only in some secretory granules. However, pre-treatment of semi-thin sections with trypsin and carboxypeptidase B led to a marked increase of Met-enkephalin immunoreactivity in B-cells. In addition, immunoreactivities for Met-enkephalin-Arg-Gly-Leu and bovine adrenal medulla dodecapeptide could be demonstrated in B- and A-cells, and beta-endorphin immunoreactivity was localized in A-cells. In no case, however, were immunoreactivities detected for bovine adrenal medulla do...Continue Reading


Dec 28, 1978·Histochemistry·D GrubeE Weber
Oct 1, 1979·The Journal of Clinical Endocrinology and Metabolism·J F BruniS S Yen
Jan 1, 1985·International Review of Neurobiology·M J Millan, A Herz
Nov 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L ScopsiL I Larsson
May 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger, N H Sternberger
Nov 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W B WatkinsS S Yen
Jan 1, 1981·Pharmacology·J C HouckC D Kimball
Mar 1, 1984·Diabetes·G I Bell, R Sanchez-Pescador
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L C LopezG F Saunders
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A S SternS Spector
Aug 11, 1980·Life Sciences·G E FeurleV Helmstaedter
Jan 1, 1983·Neuropeptides·S Spampinato, A Goldstein


Jan 1, 1992·Journal of Neural Transmission. General Section·A BerggrenU Sillén
Aug 1, 1991·Respiration Physiology·S C ScottI R Moss
Apr 1, 1993·Respiration Physiology·I R MossJ D Inman
Jul 20, 2002·European Journal of Pharmacology·María José García-BarradoJulio Moratinos
Jan 1, 1992·Acta histochemica·H H von DorscheK Fält
Feb 18, 2009·Molecular Endocrinology·Ting WenJohn E Pintar
Sep 3, 2009·World Journal of Gastroenterology : WJG·Tao-Lai QianYing Lu
Apr 15, 2000·General and Comparative Endocrinology·J C EtayoA C Villaro
Dec 1, 1992·Molecular and Cellular Neurosciences·M ZhangR L Schleicher
Jul 1, 1993·Trends in Endocrinology and Metabolism : TEM·A N Margioris
Sep 6, 2005·Molecular Genetics and Metabolism·Carla J GallagherMichèle M Sale
Aug 5, 2000·Anatomia, histologia, embryologia·T MyojinJ Yamada

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