Immunohistochemistry with anti-calbindin and anti-neurofilament antibodies in the cerebellum of methylazoxymethanol-treated mice

Journal of Neuroscience Research
Jean De Barry, G Gombos

Abstract

Mice pups were injected with methylazoxymethanol at birth (MAM0) or on the fifth postnatal day (MAM5) and their cerebella were examined when adult. Immunohistochemistry with an antiserum directed against calbindin, a protein specific for Purkinje cells, was used to survey more easily Purkinje cell position and orientation. For a general view of basket cell axon distribution, we used a monoclonal antibody that recognized the phosphorylated form of the 200 kD constituent protein of neurofilaments, which is axon specific. The present results confirm that in MAM5 the cytoarchitecture was preserved, some Purkinje cells degenerated, and the pericellular basket around the Purkinje cells was apparently normal. In MAM0 animals, the Purkinje cells appeared malpositioned and disoriented, the pinceau around the Purkinje cell hillock was absent, but basket cell axons were present. This indicated that the absence of pinceau was not due to the absence of basket cells, but probably to alterations of cell interactions, which hindered the proper pericellular basket formation.

References

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Citations

Nov 1, 1994·Neurotoxicology and Teratology·P Sullivan-JonesR R Holson
Jun 17, 2016·The Journal of Pharmacology and Experimental Therapeutics·Allison BradburyCharles Vite
Aug 30, 2018·The Journal of Comparative Neurology·Rong-Jun NiJiang-Ning Zhou
Jan 22, 1996·The Journal of Comparative Neurology·B M RiedererR A Marugg
May 15, 1995·The Journal of Comparative Neurology·D L TolbertM C La Regina

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