PMID: 8597857Sep 1, 1995Paper

Immunohistologic quantification of Pseudomonas aeruginosa in the tracheobronchial tree from patients with cystic fibrosis

Pediatric Pathology & Laboratory Medicine : Journal of the Society for Pediatric Pathology, Affiliated with the International Paediatric Pathology Association
S B PottsA Spock

Abstract

Pseudomonas aeruginosa has been recognized as a pathogen of major importance in the patient with cystic fibrosis (CF). However, no information is available regarding the histologic quantification of P. aeruginosa organisms in the CF tracheobronchial tree. We retrieved all formalin-fixed paraffin-embedded lung blocks from 20 consecutive autopsies of cystic fibrosis patients. Serial histologic sections were made and stained by three methods: hematoxylin and eosin, immunoperoxidase with anti-P. aeruginosa rabbit serum as the primary antibody, and immunoperoxidase with normal rabbit serum as the primary antibody. By studying the hematoxylin and eosin section, we classified five areas in the lung as bronchi, large bronchioles, small bronchioles, bronchioloectatic areas, and abscess/airways with destroyed epithelium. The areas stained by an anti-P. aeruginosa immunoperoxidase method were examined under high-power magnification, and the bacteria within random fields were counted. Pseudomonas aeruginosa organisms were identified in 14 of 20 cases, including 13 of 16 cases in which P. aeruginosa was specifically cultured at autopsy. Quantification of organisms within the lumens of all five airway types showed that the bacterial density ...Continue Reading

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Citations

Mar 28, 1998·American Journal of Respiratory and Critical Care Medicine·L V MöllerL van Alphen
Aug 1, 2007·Expert Review of Respiratory Medicine·Patricia J Dubin, Jay K Kolls
May 21, 2014·Advanced Drug Delivery Reviews·Ivana d'AngeloFrancesca Ungaro
May 7, 2009·Pediatric Pulmonology·Thomas BjarnsholtNiels Høiby
Nov 18, 2003·British Journal of Clinical Pharmacology·N R Labiris, M B Dolovich
Apr 29, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·B MarthaP Chavanet
Oct 7, 2003·Annual Review of Microbiology·Matthew R Parsek, Pradeep K Singh
Mar 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Thomas Bjarnsholt, Michael Givskov
Mar 26, 2004·Nature Reviews. Microbiology·Luanne Hall-StoodleyPaul Stoodley
Aug 10, 2001·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·F FaurissonP Jelazko
Oct 21, 2005·American Journal of Physiology. Cell Physiology·Agnieszka Swiatecka-UrbanBruce A Stanton

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