Immunohistological demonstration of feline infectious peritonitis virus antigen in paraffin-embedded tissues using feline ascites or murine monoclonal antibodies

Veterinary Immunology and Immunopathology
R TammerManfred Reinacher

Abstract

Feline infectious peritonitis (FIP) virus antigen was demonstrated after methanol, ethanol or formalin fixation in paraffin-embedded tissues by means of monoclonal and polyclonal antibodies. The monoclonal antibody was induced by immunization with transmissible gastroenteritis virus. Polyclonal antibodies were obtained by purification on protein A-Sepharose of ascites fluid from a cat with FIP. Almost all cats diagnosed as suffering from FIP by postmortem and histological examination exhibited FIP virus (FIPV) antigen in macrophages in granulomas whereas FIPV antigen was only once demonstrable in another location.

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Citations

May 5, 2004·Journal of Feline Medicine and Surgery·S PaltrinieriG Sironi
Mar 4, 2009·Journal of Feline Medicine and Surgery·Niels C Pedersen
Jun 2, 2009·Journal of Feline Medicine and Surgery·Diane AddieMarian C Horzinek
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Dec 25, 2015·Journal of Feline Medicine and Surgery·Sandra FeltenKatrin Hartmann
Mar 14, 2000·Veterinary Pathology·A KiparM Reinacher
Jun 8, 2013·Journal of Feline Medicine and Surgery·Edward J IvesFrancesco Cian
Mar 9, 2012·Journal of Feline Medicine and Surgery·Kate A WorthingJacqueline M Norris
May 16, 2018·The Journal of Small Animal Practice·A GiulianoF Costantino-Casas
Dec 18, 2018·Journal of Feline Medicine and Surgery·Alla KönigBianka S Schulz
Jan 30, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Sandra FeltenKaspar Matiasek
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Oct 18, 2007·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Anand RamanathanGaya Prasad
Jun 11, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Laura SanglKatrin Hartmann
Dec 9, 2003·Journal of Veterinary Internal Medicine·Katrin HartmannWalter Hermanns
Nov 17, 2019·Viruses·Sandra Felten, Katrin Hartmann
Jan 23, 2004·Journal of the American Animal Hospital Association·Nathan L BailiffGerald V Ling
Nov 17, 2020·Frontiers in Veterinary Science·Peter J Dickinson

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