Immunohistological studies of complement activation after xenogeneic perfusion of a working heart model

Transplant International : Official Journal of the European Society for Organ Transplantation
M M SuckfüllC Hammer

Abstract

Transplantation of organs from one species to another leads to immediate hyperacute rejection. Activation of complement is one important factor involved in this process. Whether complement activation is induced by preformed natural antibodies (PNAbs) via the classical pathway or by an "activator surface" via the alternative pathway is unclear. In order to simulate the relevant clinical situation of animal donor/human recipient we perfused working porcine hearts ex vivo with human blood. This also offered the possibility to study the process of complement activation in a precisely defined system with human complement proteins. PNAb titer and complement lytic activity of the plasma were measured. Immunohistological stainings for IgG, IgM, C1q, C4, C3d, C5-9, factor B, and properdin were performed on tissue sections of the left ventricle. PNAb titer almost totally disappeared within the first 5 min of perfusion. Complement lytic activity of the classical pathway decreased similarly within the first 3 h of xenogeneic and autologous perfusion from 70% to 40%. More detailed immunohistological studies revealed positive staining for C3d on endothelium and myocardium of ex vivo perfused xenogeneic hearts. Complement-induced cytotoxicity...Continue Reading

References

Jan 1, 1992·International Archives of Allergy and Immunology·D J White
Jul 1, 1988·Transplantation·H Auchincloss
Jan 1, 1971·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·W LandW Brendel

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Citations

Oct 21, 1999·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·T M BehrC E Angermann
Dec 28, 1999·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·P BrennerB Reichart

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