Immunohistostaining assays for detection of Chlamydia pneumoniae in atherosclerotic arteries indicate cross-reactions with nonchlamydial plaque constituents

Journal of Clinical Microbiology
V Y HoymansM Ieven

Abstract

Detection of Chlamydia pneumoniae antigens in PCR-negative atheromata by immunohistochemistry assays has given rise to controversies regarding a link between the bacterium and atherosclerosis. One hundred ninety-seven human arterial segments removed surgically were examined for C. pneumoniae DNA by conventional PCR with three different primer pairs and by real-time PCR in two different laboratories. No C. pneumoniae DNA was detected. Eighty atherosclerotic lesions were studied by immunohistochemistry assays. Immunoreactivity for C. pneumoniae was frequently present but was not related to the extent of atherosclerosis. Mammary arteries showed immunoreactivity. Serial sections of 17 atheromata were analyzed by Western blotting, histological staining, and UV fluorescence microscopy. Chlamydial proteins were not detected. The sites with positive results by C. pneumoniae immunohistostaining assays precisely matched the sites with autofluorescent ceroid deposits. Immunoblotting and antigenic staining for C. pneumoniae were negative in tests with fetal aortas. The absence of C. pneumoniae DNA in human atherosclerotic lesions, together with negative results for C. pneumoniae proteins by Western blotting analysis, and the perfect matchi...Continue Reading

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Citations

Jan 7, 2005·Journal of Clinical Microbiology·Margareta M Ieven, Vicky Y Hoymans
Nov 28, 2007·Annual Review of Pathology·Gustavo Valbuena, David H Walker
Jun 5, 2012·Journal of the American Veterinary Medical Association·Anthony A PilnyRoy D Berghaus
Jan 20, 2009·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Pirkka VikatmaaPekka Saikku
Mar 27, 2007·International Journal of Cardiology·Vicky Y HoymansChristiaan J Vrints
May 21, 2008·European Journal of Clinical Investigation·K KupreishviliH W M Niessen
Nov 30, 2011·Pathology·Emily A Van Vré, Hidde Bult
Oct 6, 2005·Atherosclerosis·Sven PoppertAndreas Essig
Oct 7, 2011·Mediators of Inflammation·Emily A Van VréHidde Bult
Apr 28, 2007·Avian Diseases·Olivier A Schenker, Richard K Hoop

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