Immunoinformatics-aided design of a potential multi-epitope peptide vaccine against Leishmania infantum

International Journal of Biological Macromolecules
Bahareh VakiliYounes Ghasemi

Abstract

Visceral leishmaniasis (VL) or kala-azar, the most severe form of the disease, is endemic in more than eighty countries across the world. To date, there is no approved vaccine against VL in the market. Recent advances in reverse vaccinology could be promising approach in designing the efficient vaccine for VL treatment. In this study, an efficient multi-epitope vaccine against Leishmania infantum, the causative agent of VL, was designed using various computational vaccinology methods. Potential immunodominant epitopes were selected from four antigenic proteins, including histone H1, sterol 24-c-methyltransferase (SMT), Leishmania-specific hypothetical protein (LiHy), and Leishmania-specific antigenic protein (LSAP). To enhance vaccine immunogenicity, two resuscitation-promoting factor of Mycobacterium tuberculosis, RpfE and RpfB, were employed as adjuvants. All the aforesaid segments were joined using proper linkers. Homology modeling, followed by refinement and validation was performed to obtain a high-quality 3D structure of designed vaccine. Docking analyses and molecular dynamics (MD) studies indicated vaccine/TLR4 complex was in the stable form during simulation time. In sum, we expect our designed vaccine is able to induc...Continue Reading

References

Mar 5, 1992·Nature·R LüthyD Eisenberg
Feb 1, 1995·Protein Engineering·A C WallaceJ M Thornton
Sep 1, 1993·Protein Science : a Publication of the Protein Society·C Colovos, T O Yeates
Sep 1, 1996·Clinics in Dermatology·P Desjeux
May 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Brian LivingstonAlessandro Sette
Aug 15, 2002·Human Immunology·Pedro A RecheEllis L Reinherz
Jan 18, 2003·Vaccine·Jeannette Adu-BobieMariagrazia Pizza
Jan 31, 2003·Proteins·Simon C LovellDavid C Richardson
May 23, 2003·Bioinformatics·Harpreet Singh, G P S Raghava
Jun 26, 2003·Nucleic Acids Research·Pingping GuanDarren R Flower
Oct 11, 2003·The Journal of Infectious Diseases·Slavica MasinaNicolas J Fasel
Dec 19, 2003·Nucleic Acids Research·Rolf ApweilerLai-Su L Yeh
Jul 1, 2004·Comparative Immunology, Microbiology and Infectious Diseases·P Desjeux
Apr 20, 2005·Trends in Parasitology·Rhea N Coler, Steven G Reed
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Prasad RallabhandiStefanie N Vogel
Jul 18, 2006·Nucleic Acids Research·Sudipto Saha, G P S Raghava
Oct 17, 2006·Parasitology Today·N GalantiG C Toro
Jan 9, 2007·BMC Bioinformatics·Irini A Doytchinova, Darren R Flower
May 23, 2007·Nucleic Acids Research·Markus Wiederstein, Manfred J Sippl
Oct 17, 2007·Nature Reviews. Microbiology·François ChappuisMarleen Boelaert
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Diego U FerreiroPeter G Wolynes
Jun 25, 2009·Bioinformatics·Christophe N MagnanPierre Baldi
Aug 15, 2009·Interdisciplinary Perspectives on Infectious Diseases·Wanderley de Souza, Juliany Cola Fernandes Rodrigues
Sep 30, 2009·Vaccine·Sylvie BertholetSteven G Reed
Oct 12, 2010·Bioinformatics·Christophe N MagnanPierre Baldi
Oct 30, 2010·Immunity·Robert L CoffmanRobert A Seder
Oct 30, 2010·Immunity·Alessandro Sette, Rino Rappuoli
Jan 12, 2011·Parasitology Research·Fabio A ColomboVera L Pereira-Chioccola
Jun 3, 2011·Clinical and Vaccine Immunology : CVI·Yasuyuki GotoSteven G Reed
Jun 15, 2011·Biotechnology Advances·Francesco Peri, Matteo Piazza
Jan 25, 2012·PLoS Neglected Tropical Diseases·Vinicio T S CoelhoEduardo A F Coelho
May 23, 2012·Nucleic Acids Research·Yohan KimBjoern Peters
May 31, 2012·Nucleic Acids Research·Michael JenikDiego U Ferreiro
Jun 14, 2012·PloS One·Jorge AlvarUNKNOWN WHO Leishmaniasis Control Team

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Citations

Nov 7, 2019·Medical Microbiology and Immunology·Bahareh VakiliGholam Reza Hatam
Nov 10, 2020·Pathogens and Global Health·Sunita YadavVikash Kumar Dubey
Aug 16, 2019·Research in Veterinary Science·Juliana M MachadoEduardo A F Coelho
Jan 20, 2021·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Maryam BehzadiNavid Nezafat
May 4, 2021·International Journal of Peptide Research and Therapeutics·Amir AtapourGholamreza Hatam

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