Mar 25, 2018

Immunoinformatics approaches to design a novel multi-epitope subunit vaccine against HIV infection

Vaccine
Rajan K PandeyVijay Kumar Prajapati

Abstract

The end goal of HIV vaccine designing requires novel strategies to elicit a strong humoral and cell-mediated immune response. The emergence of drug resistance and the requirement of next line treatment necessitate the finding of the potential and immunogenic vaccine candidate. This study employed a novel immunoinformatics approach to design multi-epitope subunit vaccine against HIV infection. Here, we designed the subunit vaccine by the combination of CTL, HTL and BCL epitopes along with suitable adjuvant and linkers. Physiochemical characterization of subunit vaccine was assessed to ensure its thermostability, theoretical PI, and amphipathic behavior. In further assessment, subunit vaccine was found to be immunogenic with the capability to generate humoral and cell-mediated immune response. Further, homology modeling and refinement was performed and the refined modeled structure was used for molecular docking with the immune receptor (TLR-3) present on lymphocyte cells. Consequently, molecular dynamics simulation ensured the molecular interaction between TLR-3 and subunit vaccine candidate. Disulfide engineering was performed by placing the cysteine residues in the region of high mobility to enhance the vaccine stability. At l...Continue Reading

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Mentioned in this Paper

Molecular Dynamics
Study
Immunologic Adjuvants
Cytotoxic T-Lymphocyte Analysis
Vaccines
Adjuvant
Homology Modeling
Humoral Immunity
HIV Infections
Protein Biosynthesis

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