PMID: 6406590Jul 1, 1983Paper

Immunolabeling of carbonic anhydrase isoenzyme C and glial fibrillary acidic protein in paraffin-embedded tissue sections of human brain and retina

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
T KumpulainenS H Nyström

Abstract

The specificities of carbonic anhydrase isoenzyme C (CA C) and glial fibrillary acidic (GFA) protein as immunocytochemical markers for different glial cell populations in human brain and retina were studied using indirect immunofluorescence and peroxidase-antiperoxidase complex methods. With antibodies against CA C, only those cerebral cells that were morphologically oligodendrocytes and Müller cells of the retina showed positive immunostaining reaction, whereas antibodies against GFA protein selectively labeled cerebral astrocytes and a part of the glial cells and fibers in the inner layers of the retina. In double labeling, when both glial cell markers were successively localized in the same cerebral tissue sections, GFA protein immunofluorescence was never found in the immunoperoxidase-stained CA C-positive cells, which further supports the oligodendrocyte-specificity of CA C in human brain.

Citations

Jan 1, 1989·Acta Neuropathologica·F MutinelliA Richard
Jan 1, 1987·Virchows Archiv. A, Pathological Anatomy and Histopathology·H D Schrøder
Apr 1, 1989·Journal of Neurocytology·M BerryJ P Wyse
Oct 1, 1986·Journal of Neurocytology·A Bignami, D Dahl
Aug 30, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·T KumpulainenL K Korhonen
Oct 14, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·A K ParkkilaH Rajaniemi
Dec 1, 1988·Experimental Eye Research·G P LewisS K Fisher
Jul 15, 1994·Brain Research. Developmental Brain Research·M J Seiler, R B Aramant
Jul 1, 1988·Journal of Neuroimmunology·C Griot, M Vandevelde
Aug 1, 1994·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J Y LénaA Rabié
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G S HagemanW S Sly
Sep 15, 2005·The American Journal of Surgical Pathology·Laszlo HegyiFederico Roncaroli
Apr 1, 1994·Neuropathology and Applied Neurobiology·M T GiordanaD Schiffer
Aug 1, 1985·Acta Physiologica Scandinavica·Y Ridderstråle, M Hanson
Jun 1, 1985·Acta Physiologica Scandinavica·E Lütjen-DrecollE H Bárány
Jan 1, 1989·The British Journal of Ophthalmology·M HaltiaT Kivelä
Aug 1, 1996·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·M J LeysJ V Odom
Mar 22, 1991·The Journal of Comparative Neurology·S L Semple-Rowland
Mar 12, 2011·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·F J ArenzanaR Arévalo
Dec 18, 2007·Brain Research·Sonia PalminielloAdam A Golabek
Dec 1, 1988·Experimental Eye Research·G P LewisS K Fisher
Jul 1, 1996·Vision Research·J M RamírezJ García-Sánchez
Jul 6, 2016·Frontiers in Neuroanatomy·Nuria Sánchez-Farías, Eva Candal
May 29, 2007·International Review of Neurobiology·Behrouz NikbinFatemeh Talebian
Dec 1, 1995·The Histochemical Journal·A K ParkkilaH Rajaniemi
Oct 8, 1985·The Journal of Comparative Neurology·J Schnitzer
Jan 1, 1987·Journal of Neuroscience Research·J R Connor, R E Fine
Jun 1, 1989·Journal of Neuroscience Research·M S GhandourR E Tashian
Jul 1, 1989·Annals of Neurology·M R Gerber, J R Connor
Jan 1, 1984·Annals of the New York Academy of Sciences·P Linser, A A Moscona

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