PMID: 9542586May 30, 1998Paper

Immunolabelling of the cytoplasm and processes of apoptotic facial motoneurons following axotomy in the neonatal rat

Acta Neuropathologica
J M GarrahJ P Rossiter

Abstract

A polyclonal antibody intended to recognize c-Jun (Oncogene Science, c-jun/AP-1, Ab-2) has previously been shown to recognize an apparently novel "apoptosis-specific protein" (ASP) in the cytoplasm of cells undergoing apoptotic cell death in vitro. We have investigated whether this antibody would also serve as a reliable marker for apoptotic motoneurons in vivo. Following transection of the left facial nerve in anesthetized neonatal rat pups, which results in over 90% death of the facial motoneurons, we performed immunohistochemistry on frozen brain stem sections with Oncogene Science Ab-1 and Ab-2 antibodies which are raised against different peptide fragments of c-Jun. While Ab-1/c-Jun labelling was seen in the nuclei of the majority of axotomized motoneurons, Ab-2/ASP immunoreactivity was present only in scattered cells, all of which had characteristic apoptotic morphology. Furthermore, Ab-2/ASP immunoreactivity was cytoplasmic and frequently included the dendrites and axons of dying neurons. Some cerebellar granule cells undergoing postnatal developmental cell death were also Ab-2/ASP positive. The time course of the number of Ab-2/ASP-labelled motoneurons corresponded relatively closely with our previous data on DNA fragme...Continue Reading

Citations

Jan 30, 2010·Cellular and Molecular Neurobiology·Hiroyuki IchikawaPatrice D Côté
Feb 23, 2000·Neuroscience·I FerrerA M Planas
Feb 5, 1999·Progress in Retinal and Eye Research·R LindenL B Chiarini
Nov 6, 2002·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Fumiaki MoriKoichi Wakabayashi
Mar 10, 2001·Journal of Neuroscience Research·A CasanovasJ E Esquerda
Jun 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Joan RiberaJosep E Esquerda
Dec 21, 2004·Brain Pathology·Georg Johannes MüllerFlemming Fryd Johansen

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