PMID: 8953691Nov 23, 1996Paper

Immunolocalisation of the prion protein (PrP) in the brains of sheep with scrapie

The Veterinary Record
J D FosterN Hunter

Abstract

Cheviot sheep from the Neuropathogenesis Unit flock were examined for PrP in brain sections using immunocytochemistry in order to aid scrapie diagnosis. Brains were collected from sheep which had been naturally or experimentally infected with scrapie and fixed in periodate-lysine-paraformaldehyde or in formalin. Immunolabelling was achieved using a monoclonal antibody (FH11) raised to the N-terminus of recombinant PrP protein. Several pre-treatments were studied in an effort to enhance PrP immunolabelling such as trypsin, formic acid and hydrated autoclaving. Trypsin was successful in highlighting PrP staining in formalin-fixed tissue. PrP staining was regularly observed in the dorsal vagus nucleus of the medulla oblongata and in the thalamus. Differences in the distribution and intensity of PrP immunostaining were apparent between the scrapie sources ME7 and SSBP/I.

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Citations

Mar 26, 2003·The American Journal of Pathology·Lynn M HerrmannKatherine I O'Rourke
Sep 27, 2000·Biochimica Et Biophysica Acta·L M HerrmannD P Knowles
Oct 3, 1999·Neuroscience Letters·S Verghese-NikolakakiT Sklaviadis
Feb 24, 2000·Journal of Comparative Pathology·S J RyderG A Wells
Jun 12, 2009·Journal of Comparative Pathology·E VidalR Bolea
Feb 27, 2007·Veterinary Immunology and Immunopathology·Mara S RocchiFrancesca Chianini
Oct 13, 2005·Journal of Virological Methods·Reza H DabaghianJonathan P Clewley
May 8, 2003·Clinics in Laboratory Medicine·Elizabeth S Williams
Apr 19, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·T V BaszlerS M Hall
Mar 29, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Francisco J SalgueroJuan M Torres
Apr 23, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Sabine O S DebeerAnna A Bencsik
Oct 5, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Olivier AndréolettiFrançois Schelcher
Jul 13, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anna A BencsikThierry G M Baron
Dec 20, 2013·Journal of Virology·Patricia Aguilar-CalvoJuan María Torres
May 3, 2000·The Veterinary Record·M M SimmonsJ W Wilesmith
Nov 15, 2002·The Veterinary Record·J J BadiolaA Vargas
Oct 22, 2002·The Journal of General Virology·Nora HunterFiona Houston
Oct 16, 2004·The Journal of General Virology·J-Y MadecT Baron
Jun 10, 1998·Journal of Clinical Microbiology·K I O'RourkeD P Knowles

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