Immunolocalization and histochemical evidence for the association of two different Arabidopsis annexins with secretion during early seedling growth and development

Planta
G B ClarkStanley J Roux

Abstract

Annexins are a multigene, multifunctional family of calcium-dependent, membrane-binding proteins found in animal and plant cells. In plants, annexins have been localized in the cytoplasm and at the cell periphery of highly secretory cell types, and in the tip region of polarly growing cells. Consequently, one proposed function for annexins in plant cells is participation in the Golgi-mediated secretion of new wall materials. In Arabidopsis, there are eight different annexin cDNAs, which share between 30% and 81% deduced amino acid sequence identity. We have used two monospecific Arabidopsis anti-annexin antibodies, raised against divergent 31-mer peptides from AnnAt1 and AnnAt2 and a previously characterized pea anti-annexin p35 antibody, for Western blot and immunolocalization studies in Arabidopsis. Western blot analyses of various Arabidopsis protein fractions showed that the two Arabidopsis antibodies are able to specifically recognize annexins in both soluble and membrane fractions. Immunofluorescence results with the three annexin antibodies show staining of secretory cells, especially at the cell periphery in developing sieve tubes, outer root cap cells, and in root hairs, consistent with previous results. In development...Continue Reading

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Citations

Apr 12, 2008·Purinergic Signalling·Collene R Jeter, Stanley J Roux
May 6, 2011·Journal of Experimental Botany·Marie BaucherDavid Pérez-Morga
Jun 2, 2009·Plant Physiology·Dorota Konopka-PostupolskaJacek Hennig
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