Immunolocalization of 11beta-hydroxysteroid dehydrogenase types 1 and 2 in rat uterus: variation across the estrous cycle and regulation by estrogen and progesterone

Endocrinology
P J BurtonB J Waddell

Abstract

Glucocorticoid hormone action in several target tissues is dependent not only on the expression of the glucocorticoid receptor, but also on that of the 11beta-hydroxysteroid dehydrogenase (11betaHSD) enzymes, 11betaHSD-1 and -2. In the uterus, glucocorticoids can exert inhibitory effects on a range of important functions, particularly in relation to the effects of estrogen. Therefore, the present study examined immunolocalization of the two 11betaHSD enzymes in the rat uterus at each stage of the estrous cycle and after ovariectomy with or without estrogen/progesterone replacement. In cycling rats 11betaHSD-1 was localized to luminal and glandular epithelial cells and to eosinophils in both the endometrial stroma and myometrium. In contrast, 11betaHSD-2 immunostaining was localized to endometrial stromal cells and myometrial cells, with no staining evident in epithelial cells or eosinophils. Immunostaining for both enzymes was cycle dependent, being maximal at proestrus and minimal at diestrus. Western blot analysis of whole uterus at proestrus showed the presence of 34- and 40-kDa immunoreactive species for 11betaHSD-1 and -2, respectively. These immunoreactive signals were almost abolished by ovariectomy, but this effect was ...Continue Reading

Citations

Apr 14, 2011·American Journal of Physiology. Endocrinology and Metabolism·Young S LeeKeith E Latham
Feb 26, 2000·European Journal of Obstetrics, Gynecology, and Reproductive Biology·A G GuninD V Nikolaev
Mar 29, 2016·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·P KonstantakouG Valsamakis
Apr 29, 2006·Gynecologic and Obstetric Investigation·Tsatsas ManolisOvadia Abulafia
Aug 10, 2006·American Journal of Physiology. Renal Physiology·Anikó Náray-Fejes-Tóth, Géza Fejes-Tóth
Aug 1, 2013·Physiological Reviews·Karen ChapmanJonathan Seckl
Jan 2, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jian ChenElise Gomez-Sanchez
Aug 18, 2005·American Journal of Reproductive Immunology : AJRI·Ozgul Muneyyirci-DelaleOvadia Abulafia

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