Immunolocalization of 3β-hydroxysteroid dehydrogenase in human adrenal cortex and in its disorders

Endocrine Pathology
Hironobu SasanoHiroshi Nagura

Abstract

Immunohistochemical localization of 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase (3β-HSD), which converts Δ5-3β-hydroxysteroids to Δ4-3-ketosteroids, was performed in the human adrenal gland and in its disorders by employing a specific antibody raised against the enzyme purified from human placenta. Immunoreactivity of 3β-HSD was present in all three cortical zones of the adrenal glands obtained at autopsy, while in surgically removed adrenal glands, immunoreactivity was dominant in the zona fasciculata (ZF), with faint immunoreactivity in the zona glomerulosa (ZG) and the zona reticularis (ZR). Intracortical localization of 3β-HSD in the adrenal glands obtained at autopsy may represent an adrenal adaptation to antemortem stress, with shifting of adrenal pregnenolone- a substrate of 3β-HSD - as well as steroid 17α-hydroxylase from adrenal androgen synthesis to glucocorticoid synthesis. In adrenocortical hyperplasia, marked immunoreactivity was observed in the ZG and outer ZF in adrenal glands with idiopathic hyperaldosteronism and in the ZF and ZR, especially in cortical micronodules, in the adrenal glands associated with Cushing's disease. In aldosteronoma and Cushing's adenoma, immunoreactivity of the enzyme was much more i...Continue Reading

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Citations

Oct 13, 1998·The Journal of Clinical Endocrinology and Metabolism·J S GellW E Rainey
Apr 10, 2017·Journal of Pediatric Endocrinology & Metabolism : JPEM·Dragan KatanićTomislav Ćuk
Feb 2, 2018·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Efterpi Tingi, Joe Ogah
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Aug 1, 1993·Trends in Endocrinology and Metabolism : TEM·J Ian Mason

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